Description

2'-O-(N-Methylanthraniloyl)ATP is a fluorescently labeled adenosine triphosphate (ATP) analog, specifically modified at the 2'-ribose position. This compound is a critical tool for researchers studying enzyme kinetics, nucleotide binding, and conformational changes in real-time. It is primarily utilized in life science research, particularly in the fields of biochemistry, molecular biology, and enzymology for high-sensitivity fluorescence-based assays.

Application

• Enzyme Kinetics Studies: As a fluorescent substrate for monitoring ATPase and kinase activities in continuous, real-time assays.
• Nucleotide-Binding Assays: For detecting and quantifying protein-nucleotide interactions using fluorescence anisotropy or polarization techniques.
• Conformational Analysis: To probe structural changes in motor proteins, helicases, and other ATP-dependent enzymes upon nucleotide binding and hydrolysis.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify inhibitors or activators of ATP-utilizing enzymes.
• Biophysical Research: Used in Förster Resonance Energy Transfer (FRET) and other single-molecule studies to understand molecular mechanics.
• Diagnostic Assay Development: Serves as a key component in developing sensitive diagnostic tests for enzymatic deficiencies or dysregulation.

Basic Information

Product Name	2'-O-(N-Methylanthraniloyl)ATP
CAS No.	130799-34-7
Molecular Formula	C24H28N6O14P3
Molecular Weight	717.44 g/mol
Synonyms	2'-O-(N-Methylanthraniloyl)adenosine-5'-triphosphate; Mant-ATP; 2'-O-(N-Methyl-2-anthraniloyl)ATP; N-Methylanthraniloyl-ATP; 2'-Mant-ATP; Fluorescent ATP analog; 2'-O-[N-(Methyl)anthraniloyl]adenosine-5'-triphosphate; ATP, 2'-O-[N-(methyl)anthraniloyl]-
EINECS	Contact for details

Quality Control

Our 2'-O-(N-Methylanthraniloyl)ATP is produced under stringent quality management protocols. Each batch is characterized and tested to ensure high purity and consistent performance in sensitive fluorescence applications. Certificates of Analysis (COA) detailing purity, fluorescence properties, and HPLC chromatograms are available upon request to support your research and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store desiccated under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Fluorescence Properties	Excitation ~360 nm, Emission ~440 nm (in aqueous buffer)
Biological Activity	Functional as substrate for representative ATPases/kinases

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.