Description

4-Methylluciferin is a chemically modified derivative of the native luciferin substrate, engineered to enhance its utility in advanced bioluminescent assays. This compound is critical for researchers and developers seeking to improve the sensitivity, stability, and versatility of reporter gene systems and other luminescence-based detection platforms. It is primarily utilized in the life sciences sector for applications in high-throughput screening, molecular imaging, and biomedical research, where precise and reliable signal generation is paramount.

Application

• Reporter Gene Assays: As a substrate for modified luciferase enzymes (e.g., firefly luciferase mutants) in gene expression studies and promoter activity analysis.
• High-Throughput Screening (HTS): Enables sensitive detection of drug candidates, enzyme inhibitors, or cellular responses in automated screening platforms.
• Bioluminescence Imaging (BLI): Used in preclinical in vivo imaging to track tumor growth, gene delivery, and cell migration in real-time.
• ATP Detection & Cell Viability Assays: Serves as a key component in kits for measuring cellular ATP levels as an indicator of metabolic activity and cytotoxicity.
• Biosensor Development: Integral to the design of novel biosensors for environmental monitoring, clinical diagnostics, and pathogen detection.
• Enzyme Kinetics Studies: Employed to study the catalytic mechanisms and efficiency of engineered luciferase enzymes.

Basic Information

Product Name	4-Methylluciferin
CAS No.	130593-26-9
Molecular Formula	C12H8N2O3S2
Molecular Weight	292.33 g/mol
Synonyms	D-Luciferin 4-methyl ether; 4-Methyl-D-luciferin; 2-(6-Hydroxy-4-methylbenzothiazol-2-yl)-2-thiazoline-4-carboxylic acid; 4-Methyl luciferin; 4-Me-Luc; 4-Methylluciferin potassium salt (common salt form); 130593-26-9 (CAS); Light-emitting substrate for luciferase
EINECS	Contact for details

Quality Control

Our 4-Methylluciferin is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation. A Certificate of Analysis (COA) detailing specifications such as purity, absorbance profile, and functional activity is provided with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccant and under an inert atmosphere to maintain stability and potency. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Bioluminescent Activity	Functional activity confirmed vs. standard
Solubility	Soluble in DMSO, buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.