Description

4-Nitrophenyl-3(5)-o-n-Acetylglucosaminyl-Maltopentaoside is a specialized chromogenic substrate designed for the sensitive and specific detection of glycosidase enzyme activity. This compound matters for researchers and quality control labs requiring precise, quantitative assays in enzymatic studies. It is primarily needed by the biotechnology, pharmaceutical, and diagnostic industries for applications in enzyme kinetics, inhibitor screening, and biochemical research.

Application

• Enzyme Activity Assays: As a chromogenic substrate for the detection and quantification of specific glycosidases, including chitinases and hexosaminidases.
• Biochemical Research: Used in kinetic studies to determine enzyme parameters such as Km and Vmax, and to study enzyme mechanisms.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential enzyme inhibitors for drug discovery.
• Diagnostic Development: Serves as a key reagent in the development of diagnostic kits for enzymatic disorders or pathogen detection.
• Quality Control: Utilized in biomanufacturing to monitor the activity and purity of enzyme preparations.
• Microbiology Studies: Applied in research to investigate the role of glycosidases in microbial pathogenesis and metabolism.

Basic Information

Product Name	4-Nitrophenyl-3(5)-o-n-Acetylglucosaminyl-Maltopentaoside
CAS No.	130342-76-6
Molecular Formula	C41H64N2O31
Molecular Weight	1056.95 g/mol
Synonyms	p-Nitrophenyl N,N'-diacetyl-β-chitopentaoside; 4-Nitrophenyl N,N'-diacetyl-β-chitopentaoside; PNP-(GlcNAc)2-5; Chitinase substrate; 4-Nitrophenyl β-D-N,N'-diacetylchitopentaoside; Hexosaminidase substrate; NAG5-pNP; 4-NP-β-(GlcNAc)5
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl-3(5)-o-n-Acetylglucosaminyl-Maltopentaoside is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.