Description

10-Hydroxyamitriptyline-n-Glucuronide is a key glucuronide metabolite of the tricyclic antidepressant amitriptyline. This compound is of significant importance in pharmaceutical research and development, particularly for pharmacokinetic studies, drug metabolism investigations, and as a certified reference standard for bioanalytical assays. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and manufacturers of diagnostic kits and reference materials for ensuring accurate quantification and regulatory compliance in clinical and forensic toxicology.

Application

• Pharmaceutical Reference Standard: Certified material for the calibration and validation of analytical methods (e.g., LC-MS/MS, HPLC) used in drug development and bioanalysis.
• Metabolite Identification & Pharmacokinetics: Critical for studying the metabolic pathways, bioavailability, and elimination profile of amitriptyline in preclinical and clinical research.
• Forensic & Clinical Toxicology: Used as a quantitative standard in assays to monitor amitriptyline and its metabolites in biological samples for therapeutic drug monitoring or overdose cases.
• Biomarker Research: Employed in studies investigating the correlation between metabolite levels and drug efficacy or adverse effects.
• Quality Control in Manufacturing: Serves as an impurity standard to ensure the purity and consistency of amitriptyline active pharmaceutical ingredient (API) batches.
• Enzyme Activity Studies: Used in vitro to study the activity of UDP-glucuronosyltransferase (UGT) enzymes responsible for its formation.

Basic Information

Item	Detail
Product Name	10-Hydroxyamitriptyline-n-Glucuronide
CAS No.	130209-87-9
Molecular Formula	C25H30N2O7
Molecular Weight	470.52 g/mol
Synonyms	10-Hydroxyamitriptyline N-Glucuronide; 10-OH-Amitriptyline N-Glucuronide; Amitriptyline Metabolite; 10-Hydroxyamitriptyline Glucuronide; (3-(10,11-Dihydro-5H-dibenzo[a,d]cyclohepten-5-ylidene)-1-(dimethylamino)propyl) β-D-glucopyranosiduronic acid; E-10-hydroxyamitriptyline glucuronide
EINECS	Contact for details

Quality Control

Our 10-Hydroxyamitriptyline-n-Glucuronide is produced and handled under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via mass spectrometry and NMR, to ensure it meets the high standards required for research and reference materials. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥95%
Water Content (KF)	≤5.0%
Solubility	Soluble in methanol, DMSO; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.