Description

Rhod-2, Am is a cell-permeant, acetoxymethyl (AM) ester derivative of the calcium indicator Rhod-2. This compound is specifically engineered for the selective and sensitive detection of intracellular calcium ions (Ca²⁺), a critical secondary messenger in numerous cellular processes. It is an essential tool for researchers in life sciences, enabling real-time monitoring of calcium dynamics in live cells. Key industries and applications include biomedical research, pharmaceutical development, and neurobiology.

Application

• Intracellular Calcium Imaging: Primary use for visualizing and quantifying Ca²⁺ flux in live cells using fluorescence microscopy.
• Neuroscience Research: Studying calcium signaling in neurons, synaptic activity, and neurotransmitter release.
• Cardiovascular Research: Monitoring calcium transients in cardiac myocytes and vascular smooth muscle cells.
• Drug Discovery & Screening: Used in high-throughput assays to evaluate compound effects on cellular calcium pathways.
• Cell Physiology Studies: Investigating calcium's role in muscle contraction, cell proliferation, and apoptosis.
• Fluorometric Assays: Employed in plate readers for quantitative measurement of calcium concentration in cell populations.

Basic Information

Item	Detail
Product Name	Rhod-2, Am
CAS No.	129787-64-0
Molecular Formula	C₄₇H₅₀Cl₂N₄O₁₉
Molecular Weight	1053.82 g/mol
Synonyms	Rhod-2 AM ester; Rhod-2 acetoxymethyl ester; Rhod-2, AM; Rhod2-AM; X-Rhod-1, AM; Calcium indicator Rhod-2 AM; 5-(and-6)-Carboxy-X-rhodamine, acetoxymethyl ester, triethylammonium salt; 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester
EINECS	Contact for details

Quality Control

Our Rhod-2, Am is produced under strict quality management protocols to ensure batch-to-batch consistency and performance reliability for critical research. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometric analysis for correct spectral properties, and functional validation in cell-based assays. A detailed Certificate of Analysis (COA) documenting identity, purity, and performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Excitation/Emission Max	~553 nm / ~581 nm (Ca²⁺ bound)
Extinction Coefficient (ε)	Contact for details
Water Content (KF)	< 1.0%
Solubility	DMSO, DMF (≥ 10 mM)
Functional Activity	Validated in cell-based calcium imaging assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.