Description

1-Methylimidazole-2-Carboxamide Netropsin is a structurally unique minor-groove DNA-binding compound derived from the netropsin family, engineered with a 1-methylimidazole-2-carboxamide substituent to enhance sequence selectivity and binding affinity. This specialty research chemical serves as a critical tool for molecular biology studies involving gene regulation, chromatin dynamics, and targeted nucleic acid interference. It is primarily utilized by pharmaceutical R&D laboratories, academic genomics centers, and biotech firms developing sequence-specific DNA modulators. Its high functional specificity makes it indispensable in probe design and mechanistic studies of transcriptional control.

Application

• Research-grade DNA minor-groove binder for studying sequence-specific protein–DNA interactions
• Tool compound in epigenetic research for probing chromatin accessibility and histone-DNA crosstalk
• Lead structure for rational design of synthetic transcription factor inhibitors
• Component in development of anti-viral or anti-cancer agents targeting DNA replication fidelity
• Reference standard in HPLC and LC-MS method validation for nucleic acid-binding small molecules
• Probe in fluorescence anisotropy and surface plasmon resonance (SPR) binding assays
• Chemical biology reagent for crosslinking studies with photoactivatable derivatives
• Quality control standard for GMP-compliant oligonucleotide synthesis facilities

Basic Information

Product Name	1-Methylimidazole-2-Carboxamide Netropsin
CAS No.	129601-50-9
Molecular Formula	C22H31N11O4
Molecular Weight	513.57 g/mol
Synonyms	1-Methylimidazole-2-carboxamide netropsin; Netropsin derivative MIM-2-C; N-(1-Methyl-1H-imidazol-2-yl)carbonyl-netropsin; 1-Methyl-2-(netropsin-4-carboxamido)imidazole; Netropsin-1-methylimidazole-2-carboxamide conjugate; MIMC-Net; 2-Carboxamido-1-methylimidazole-netropsin; Netropsin-MIM
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. All batches conform to stringent analytical protocols including HPLC purity verification, NMR structural confirmation, residual solvent analysis (GC), and heavy metal screening (ICP-MS). The material meets specifications appropriate for research-grade biochemical applications and is manufactured under ISO 9001-certified quality management systems.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, maintain in low-humidity conditions (<30% RH) and minimize exposure to ambient air during handling.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.5%
Related Substances (HPLC)	≤1.0% total impurities
Residual Solvents (GC)	Meets ICH Q3C Class 3 limits
Water Content (KF)	≤0.5%
Heavy Metals (as Pb)	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.