Description

5-Bromo-4-Chloro-3-Indolyl-n-Acetyl-β-D-Galactosaminide is a specialized chromogenic substrate used for the detection of N-acetyl-β-D-galactosaminidase (NAGase) activity. This compound matters for its high sensitivity and specificity in enzymatic assays, providing a clear, insoluble blue-green precipitate upon cleavage. It is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic kit manufacturing sectors who require reliable tools for enzyme-linked detection and histochemical staining.

Application

• Enzymatic Assay Substrate: Primary substrate for detecting and quantifying N-acetyl-β-D-galactosaminidase (NAGase) activity in research and diagnostic applications.
• Diagnostic Kit Manufacturing: A key component in the production of diagnostic kits for lysosomal storage disorders and other conditions linked to NAGase levels.
• Microbiology & Cell Biology Research: Used in histochemistry and cytochemistry for visualizing enzyme activity in tissue sections, cell cultures, and bacterial colonies (e.g., in blue-white screening with modified vectors).
• Biochemical Research: Employed in enzyme kinetics studies and inhibitor screening to understand NAGase function and regulation.
• Pharmaceutical Quality Control: Utilized in potency testing and stability studies of enzyme-based therapeutics.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl-n-Acetyl-β-D-Galactosaminide
CAS No.	129572-48-1
Molecular Formula	C16H18BrClN2O6
Molecular Weight	449.68 g/mol
Synonyms	X-N-Acetyl-β-D-galactosaminide; 5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-galactosaminide; NAGase Substrate; BCIP-NAG; GalNAc-β-5-Br-4-Cl-3-indolyl; 3-Indolyl GalNAc; 5-Br-4-Cl-3-Indoxyl N-acetyl-β-D-galactosaminide
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl-n-Acetyl-β-D-Galactosaminide is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.