Description

5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide Sodium Salt is a chromogenic substrate widely used in molecular biology and diagnostic assays. This compound is critical for the specific detection of the β-glucuronidase (GUS) enzyme, serving as a key reagent in reporter gene systems and microbiological testing. It is an essential component for researchers and manufacturers in the biotechnology, pharmaceutical, and clinical diagnostics industries who require reliable and sensitive enzymatic detection.

Application

• GUS Reporter Gene Assays: Primary substrate for visualizing and quantifying β-glucuronidase activity in plant molecular biology, transgenic research, and promoter studies.
• Microbiological Identification: Used in selective media for the detection of E. coli and other coliform bacteria that express GUS, crucial for food safety and water quality testing.
• Histochemistry & Cell Staining: Enables precise localization of GUS enzyme activity in tissue sections and cell cultures, forming an insoluble blue indigo dye upon cleavage.
• Diagnostic Kit Manufacturing: A key raw material in the production of diagnostic kits and reagents for clinical and environmental microbiology.
• Enzyme Activity Studies: Employed in biochemical research to study enzyme kinetics, inhibition, and function of β-glucuronidase.
• Pharmaceutical Research: Used in high-throughput screening (HTS) assays to identify modulators of GUS activity for drug discovery.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide Sodium Salt
CAS No.	129541-41-9
Molecular Formula	C14H13BrClNNaO7
Molecular Weight	454.60 g/mol
Synonyms	X-Glucuronide Sodium Salt; 5-Bromo-4-chloro-3-indolyl-β-D-glucopyranosiduronic acid sodium salt; BCIG; X-GlcA Na; X-β-D-Glucuronide; 5-Bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid sodium salt; X-GUS Substrate
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide Sodium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Heavy Metals	< 20 ppm
Biological Activity	Suitable for GUS assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.