Description

p-Nitrophenyl β-D-Cellopentaoside, Hexadecaacetate is a specialized chromogenic substrate used for the sensitive and quantitative assay of cellulase enzyme activity. This compound is critical for researchers and quality control laboratories requiring precise measurement of enzymatic hydrolysis in biomass conversion and related bioprocesses. It is primarily utilized in the biotechnology, biofuel research, and enzyme manufacturing industries for analytical and diagnostic applications.

Application

• Cellulase Enzyme Assays: Primary substrate for the spectrophotometric determination of endo- and exo-cellulase activity in research and industrial samples.
• Biomass Conversion Research: Used to study and optimize enzymatic hydrolysis processes for biofuel (e.g., cellulosic ethanol) production.
• Enzyme Kinetics & Characterization: Essential tool for investigating the mechanism, specificity, and inhibition of cellulolytic enzymes.
• Quality Control in Enzyme Production: Employed by manufacturers to standardize and certify the activity of commercial cellulase preparations.
• Microbiological & Agricultural Research: Applied in studies of plant-pathogen interactions and the screening of microorganisms for cellulase production.
• Diagnostic Kits & Reagents: Serves as a key component in formulated test kits for environmental monitoring and industrial process control.

Basic Information

Product Name	p-Nitrophenyl β-D-Cellopentaoside, Hexadecaacetate
CAS No.	129411-66-1
Molecular Formula	C76H91NO43
Molecular Weight	1690.52 g/mol
Synonyms	4-Nitrophenyl β-D-cellopentaoside hexadecaacetate; pNP-Cellopentaose hexadecaacetate; Chromogenic cellulase substrate; 4-Nitrophenyl β-cellopentaoside peracetate; 1,2,3,4,6-Penta-O-acetyl-β-D-glucopyranosyl-(1→4)-O-2,3,6-tri-O-acetyl-β-D-glucopyranosyl-(1→4)-O-2,3,6-tri-O-acetyl-β-D-glucopyranosyl-(1→4)-O-2,3,6-tri-O-acetyl-β-D-glucopyranosyl-(1→4)-O-2,3,6-tri-O-acetyl-β-D-glucopyranoside 1-(4-nitrophenyl); PNPC
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl β-D-Cellopentaoside, Hexadecaacetate is manufactured under strict quality systems to ensure high purity and consistent performance for critical enzymatic assays. Each batch is thoroughly characterized using advanced analytical techniques, including HPLC and NMR, to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing assay, impurity profile, and physical specifications is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, acetone; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.