Description

p-Nitrophenyl β-D-Cellopentaoside is a specialized chromogenic substrate used for the precise quantification of cellulase enzyme activity. This compound matters because it provides a highly sensitive and specific method for analyzing endo-1,4-β-glucanase activity, a critical parameter in industrial enzyme research and quality control. Industries that need it include biotechnology firms, academic research institutions, and manufacturers involved in biofuel production, textile processing, and animal feed formulation, where monitoring cellulolytic activity is essential for process optimization and product development.

Application

• Cellulase Enzyme Assays: Primary substrate for the colorimetric determination of endo-1,4-β-glucanase (cellulase) activity in research and industrial QC labs.
• Biofuel Research: Used to screen and characterize novel cellulase enzymes for the efficient saccharification of lignocellulosic biomass into fermentable sugars.
• Textile & Paper Industry: Employed in the development and potency testing of enzymatic formulations used for biostoning denim and biopolishing fabrics or modifying pulp.
• Animal Feed Additive Development: Facilitates the activity measurement of cellulase enzymes added to feed to improve digestibility and nutrient availability.
• Microbiological & Fungal Studies: Serves as a diagnostic tool to study cellulase production and secretion in microorganisms like Trichoderma and Aspergillus species.
• Enzyme Purification & Characterization: A critical reagent for monitoring specific activity during enzyme purification processes and kinetic studies.
• Quality Control for Industrial Enzymes: Essential for batch-to-batch consistency testing of commercial cellulase preparations supplied to various manufacturing sectors.
• Diagnostic Kit Manufacturing: Used as a key component in the formulation of diagnostic kits and reagents for enzymatic analysis in food and agricultural testing.

Basic Information

Product Name	p-Nitrophenyl β-D-Cellopentaoside
CAS No.	129411-63-8
Molecular Formula	C41H55NO31
Molecular Weight	1033.87 g/mol
Synonyms	4-Nitrophenyl β-D-cellopentaoside; pNP-Cellopentaose; p-Nitrophenyl β-cellopentaoside; 4-Nitrophenyl β-cellopentaoside; Cellopentaose 4-nitrophenyl β-D-glycoside; p-Nitrophenyl-β-D-cellopentaose; 1,4-β-D-Cellopentaosyl 4-nitrophenyl β-D-glucopyranoside; PNPC5
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl β-D-Cellopentaoside is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency suitable for sensitive enzymatic assays. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which may affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO
Enzymatic Suitability	Passes performance test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.