Description

p-Nitrophenyl B-D-Cellotetraoside is a chromogenic substrate specifically designed for the detection and quantification of cellulase enzyme activity. This compound is critical for research and industrial applications where precise measurement of cellulose-degrading enzymes is required. It is primarily utilized by biotechnologists, enzymologists, and quality control laboratories in the biofuel, pulp & paper, textile, and animal feed industries to assess enzymatic performance and optimize processes.

Application

• Cellulase Enzyme Assays: As a specific chromogenic substrate for the colorimetric determination of endo-1,4-β-glucanase (cellulase) activity in research and industrial samples.
• Biomass Conversion Research: Used in enzymatic hydrolysis studies to evaluate the efficiency of cellulase cocktails for biofuel (e.g., ethanol) production from lignocellulosic biomass.
• Quality Control in Enzyme Production: Employed by manufacturers to standardize and certify the activity of commercial cellulase preparations for various industrial processes.
• Textile and Pulp & Paper Industries: For monitoring cellulase enzyme activity in bio-polishing of textiles and enzymatic deinking/refining of pulp.
• Animal Feed Additive Analysis: To assay cellulase enzymes used as feed additives to improve digestibility in monogastric animals.
• Diagnostic Kit Development: Serves as a key component in the development of diagnostic kits and reagents for enzymatic analysis in clinical and environmental microbiology.

Basic Information

Product Name	p-Nitrophenyl B-D-Cellotetraoside
CAS No.	129411-62-7
Molecular Formula	C30H43NO22
Molecular Weight	745.66 g/mol
Synonyms	4-Nitrophenyl β-D-cellotetraoside; pNP-Cellotetraoside; 4-Nitrophenyl β-cellotetraoside; p-Nitrophenyl β-cellotetraoside; Cellotetraose 4-nitrophenyl β-D-glycoside; PNPC; 4-NPC; 1,4-β-D-Cellotetraosyl 4-nitrophenyl ether
EINECS	Contact for details

Quality Control

Every batch of p-Nitrophenyl B-D-Cellotetraoside is produced and tested under a strict quality management system. We ensure high purity and consistent enzymatic activity suitability through advanced analytical techniques. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and assay performance is provided with each shipment to guarantee it meets the exacting standards required for research and industrial enzyme analysis.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Specific Optical Rotation	Contact for details
Enzymatic Suitability	Suitable for cellulase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.