Description

5-Azido-2-Nitrobenzoyl-8-Azido Atp is a specialized, bifunctional photoactivatable ATP analog designed for advanced biochemical research and probe development. This compound matters for its unique ability to act as a cross-linking agent upon UV irradiation, enabling the study and mapping of ATP-binding sites within proteins and enzymes. Researchers and developers in the fields of chemical biology, proteomics, and medicinal chemistry need this high-purity reagent for creating affinity labels, developing diagnostic assays, and investigating nucleotide-protein interactions.

Application

• Photoaffinity Labeling: A critical tool for covalently tagging and identifying ATP-binding proteins and active sites in complex biological systems.
• Enzyme Mechanism Studies: Used to probe the structure and function of kinases, ATPases, and other nucleotide-dependent enzymes.
• Bioconjugation & Probe Synthesis: Serves as a key intermediate for synthesizing advanced molecular probes and diagnostic conjugates.
• Proteomic Research: Facilitates the discovery and validation of novel drug targets by mapping protein-ligand interactions.
• Medicinal Chemistry: Employed in the design and development of novel enzyme inhibitors and therapeutic agents.
• Diagnostic Assay Development: Used to create sensitive and specific detection systems for clinical and research applications.

Basic Information

Product Name	5-Azido-2-Nitrobenzoyl-8-Azido Atp
CAS No.	129391-97-5
Molecular Formula	C₁₇H₁₈N₁₀O₁₃P₃
Molecular Weight	672.33 g/mol
Synonyms	8-Azido-adenosine-5'-triphosphate, 2-Nitro-5-azidobenzoyl ester; 2-Nitro-5-azidobenzoyl-8-azido-ATP; NAB-8-N₃-ATP; 5'-O-[N-(2-Nitro-5-azidobenzoyl)]-8-azidoadenosine triphosphate; Bifunctional Photoactivatable ATP Analog; 8-N₃-ATP-5'-NAB; ATP Cross-linking Reagent
EINECS	Contact for details

Quality Control

Every batch of 5-Azido-2-Nitrobenzoyl-8-Azido Atp is manufactured and analyzed under stringent quality systems to ensure the high purity and consistency required for sensitive research applications. Our products undergo rigorous quality testing, including HPLC, NMR, and MS analysis, to ensure compliance with exacting standards. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability is provided with each shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to warm to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.