Description

Succinimidyl-Alanyl-Phenylalanyl-Prolyl-Phenylalanine 4-Nitroanilide is a specialized synthetic peptide derivative designed as a chromogenic substrate for protease activity assays. This compound matters for its high specificity and sensitivity in detecting and quantifying enzymatic activity, particularly for serine and cysteine proteases. It is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors for drug discovery, biochemical research, and enzyme characterization.

Application

• Protease Activity Assays: Used as a chromogenic substrate to measure the kinetic activity of specific proteolytic enzymes in vitro.
• Drug Discovery & Screening: Employed in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitors as therapeutic candidates.
• Biochemical Research: A critical tool for studying enzyme kinetics, mechanism of action, and substrate specificity of proteases.
• Diagnostic Kit Development: Serves as a key component in the development of diagnostic assays for diseases linked to abnormal protease activity.
• Quality Control in Biomanufacturing: Used to monitor and validate the activity and consistency of enzyme batches used in production processes.

Basic Information

Product Name	Succinimidyl-Alanyl-Phenylalanyl-Prolyl-Phenylalanine 4-Nitroanilide
CAS No.	128802-73-3
Molecular Formula	C36H39N7O9
Molecular Weight	713.74 g/mol
Synonyms	Succinyl-Ala-Phe-Pro-Phe-pNA; Suc-AFPF-pNA; Protease Substrate Suc-AFPF-pNA; N-Succinimidyl-Ala-Phe-Pro-Phe-4-nitroanilide; 4-Nitroanilide of Succinyl-Alanyl-Phenylalanyl-Prolyl-Phenylalanine; Chromogenic Protease Substrate; Peptidyl 4-Nitroanilide Substrate
EINECS	Contact for details

Quality Control

Our Succinimidyl-Alanyl-Phenylalanyl-Prolyl-Phenylalanine 4-Nitroanilide is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (Mass Spectrometry)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.