Description

Tos-Gly-Pro-Lys-Amc Acoh is a specialized fluorogenic peptide substrate designed for protease activity assays. This compound matters for researchers and quality control laboratories requiring precise, sensitive, and reliable measurement of enzymatic activity, particularly for trypsin-like proteases. It is primarily needed by the pharmaceutical, biotechnology, and life science research sectors for drug discovery, biochemical research, and diagnostic development.

Application

• Protease Activity Assays: Primary substrate for the fluorometric detection and quantification of trypsin, plasmin, thrombin, and related serine proteases.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize protease inhibitors for therapeutic development.
• Biochemical Research: Essential tool for studying protease kinetics, enzyme specificity, and catalytic mechanisms in vitro.
• Diagnostic Development: Employed in the development of clinical assays to measure protease levels in biological samples.
• Quality Control in Biologics: Used to monitor and validate the activity of enzyme-based pharmaceuticals and recombinant proteins.

Basic Information

Product Name	Tos-Gly-Pro-Lys-Amc Acoh
CAS No.	128202-25-5
Molecular Formula	C31H39N7O8S
Molecular Weight	669.75 g/mol
Synonyms	Tosyl-Glycyl-Prolyl-Lysyl-7-Amino-4-Methylcoumarin Acetate; Tos-Gly-Pro-Lys-AMC Acetate; Nα-Tosyl-Gly-Pro-Lys-AMC Acetate; Chromogenic Substrate for Trypsin; Boc-Gly-Pro-Arg-MCA (related class); Fluorogenic Peptide Substrate 128202-25-5; Trypsin Substrate IV (AMC); H-Tos-Gly-Pro-Lys-AMC•AcOH
EINECS	Contact for details

Quality Control

Our Tos-Gly-Pro-Lys-Amc Acoh is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to expected kinetic parameters for trypsin

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.