Description

2'-Deoxy-3'-o-(N'-Methylanthraniloyl)Adenosine-5'-o-Triphosphate (Mant-dATP) is a fluorescently labeled nucleotide analog, a critical reagent for advanced molecular biology and biochemistry research. Its core value lies in its ability to act as a chain terminator in DNA sequencing and other enzymatic assays, where its fluorescent tag allows for high-sensitivity detection and real-time monitoring of polymerase activity. This compound is essential for researchers in genomics, diagnostics, and enzymology who require precise, fluorescent-based detection methods for nucleic acid analysis and enzyme kinetics studies.

Application

• DNA Sequencing: Used as a fluorescent chain-terminating substrate in Sanger sequencing and next-generation sequencing platforms.
• Enzyme Kinetics Studies: Employed as a substrate to monitor the activity of DNA polymerases, reverse transcriptases, and other nucleotide-processing enzymes in real-time.
• Single-Molecule Detection: Facilitates the visualization and study of DNA synthesis and protein-DNA interactions at the single-molecule level.
• Diagnostic Assay Development: Serves as a key component in the development of fluorescent-based diagnostic kits for pathogen detection and genetic analysis.
• High-Throughput Screening (HTS): Utilized in assays to screen for inhibitors or activators of polymerase activity in drug discovery.
• Apoptosis Detection (TUNEL Assay): Can be used in modified TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) assays for detecting DNA fragmentation.

Basic Information

Product Name	2'-Deoxy-3'-o-(N'-Methylanthraniloyl)Adenosine-5'-o-Triphosphate (Mant-dATP)
CAS No.	128113-53-1
Molecular Formula	C27H30N6O15P3
Molecular Weight	804.49 g/mol
Synonyms	Mant-dATP; N-Methylanthraniloyl-dATP; 2'-Deoxyadenosine-5'-triphosphate, 3'-O-[N-(methylanthraniloyl)]-; 3'-O-(N-Methyl-2-anthraniloyl)-2'-deoxyadenosine-5'-triphosphate; 3'-O-(N-Methylanthraniloyl)-2'-deoxyadenosine-5'-triphosphate; Fluorescent dATP; 2′-Deoxy-3′-O—(N-methylanthraniloyl)adenosine-5′-O-triphosphate
EINECS	Contact for details

Quality Control

Our Mant-dATP is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and spectroscopic verification of the fluorescent label. Certificates of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized solid or solution
Identification (HPLC)	Retention time corresponds to reference standard
Identification (UV-Vis)	Spectrum consistent with Mant-dATP profile
Purity (HPLC)	≥ 95%
Fluorescence Properties	Excitation ~356 nm, Emission ~448 nm (in aqueous buffer)
Water Content (Karl Fischer)	≤ 5.0 %
Enzymatic Incorporation Test	Suitable for use as a substrate for specific polymerases

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.