Description

4-Trifluoromethylumbelliferyl Iduronide is a specialized fluorogenic substrate designed for the sensitive detection and quantification of α-L-iduronidase enzyme activity. This compound matters for its critical role in biochemical research and diagnostic applications, particularly in the study of lysosomal storage disorders such as Mucopolysaccharidosis I (Hurler, Scheie, and Hurler-Scheie syndromes). It is primarily needed by research institutions, diagnostic laboratories, and pharmaceutical companies engaged in enzyme kinetics studies, high-throughput screening for therapeutic agents, and the development of clinical diagnostic assays.

Application

• Enzyme Activity Assays: Primary fluorogenic substrate for the specific and sensitive measurement of α-L-iduronidase activity in vitro.
• Diagnostic Development: Key reagent in the development of clinical diagnostic kits for the detection of Mucopolysaccharidosis I (MPS I).
• Biochemical Research: Used in research laboratories to study the kinetics, inhibition, and function of the α-L-iduronidase enzyme.
• Drug Discovery Screening: Employed in high-throughput screening (HTS) platforms to identify potential pharmacological chaperones or enzyme replacement therapies for lysosomal storage diseases.
• Cell-Based Studies: Utilized in assays to measure enzymatic activity within cell lysates or cultured cells for functional genomics and pathology studies.
• Quality Control in Biomanufacturing: Acts as a critical quality control tool for processes involving recombinant α-L-iduronidase production.

Basic Information

Product Name	4-Trifluoromethylumbelliferyl Iduronide
CAS No.	128095-54-5
Molecular Formula	C₁₆H₁₅F₃O₈
Molecular Weight	392.28 g/mol
Synonyms	4-MU-α-L-Iduronide; 4-Methylumbelliferyl-α-L-iduronide (trifluoromethyl analog); α-L-Iduronide 4-trifluoromethylumbelliferyl ester; 4-Trifluoromethylumbelliferyl-α-L-iduronide; TFMU-Iduronide; Fluorogenic iduronide substrate; Iduronidase substrate; 4-Trifluoromethyl-7-hydroxycoumarin α-L-iduronide
EINECS	Contact for details

Quality Control

Our 4-Trifluoromethylumbelliferyl Iduronide is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzyme assays. We adhere to relevant quality guidelines to support our customers' compliance needs in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Optical Rotation	Contact for details
Functional Activity (Enzyme Assay)	Conforms to reference standard
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.