Description

Veratryl Tetrazolium Blue is a specialized tetrazolium salt derivative used as a vital redox indicator and chromogenic substrate in biochemical assays. Its core value lies in its ability to produce a distinct, water-insoluble formazan dye upon reduction, enabling the sensitive detection and quantification of enzymatic activity. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, particularly for applications involving dehydrogenase enzymes and cell viability testing.

Application

• Primary substrate for the detection and assay of dehydrogenase enzyme activity in biochemical research.
• Chromogenic agent in cell viability and cytotoxicity assays (e.g., MTT assay alternatives) for drug discovery.
• Redox indicator in microbiological media and diagnostic kits for metabolic activity measurement.
• Visualization reagent in histochemistry and blotting techniques (e.g., Western, Southern) for enzyme-linked detection.
• Research tool for studying mitochondrial function and cellular respiration pathways.
• Quality control testing in pharmaceutical manufacturing for sterility and microbial limit tests.
• Component in biosensor development for environmental monitoring and clinical diagnostics.

Basic Information

Product Name	Veratryl Tetrazolium Blue
CAS No.	127615-65-0
Molecular Formula	C₂₀H₂₂N₈O₆
Molecular Weight	446.44 g/mol
Synonyms	VTB; 2,2'-(3,3'-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis(3,5-diphenyl-2H-tetrazolium) Dichloride; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylylene)bis[2,5-diphenyl-2H-tetrazolium] Dichloride; Veratrole Blue Tetrazolium; Tetrazolium Blue Veratryl Derivative; BTT; Veratryl Blue TT
EINECS	Contact for details

Quality Control

Our Veratryl Tetrazolium Blue is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous analytical testing, including HPLC for purity and spectrophotometric verification of chromogenic properties. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and performance specifications is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Absorbance Spectrum	Conforms to reference
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.