Description

p-Anisyl Blue Tetrazolium Chloride is a specialized tetrazolium salt derivative used as a vital chromogenic reagent in biochemical and histochemical staining. This compound matters for its ability to produce a distinct, insoluble blue formazan precipitate upon enzymatic reduction, making it a critical tool for visualizing cellular metabolic activity. It is primarily needed by researchers and quality control laboratories in the life sciences, pharmaceutical development, and diagnostic manufacturing sectors for applications such as cell viability assays and enzyme-linked detection.

Application

• Cell Viability and Cytotoxicity Assays: Used as a substrate to quantify metabolically active cells, commonly in drug screening and toxicology studies.
• Histochemical Staining: Employed for the localization of dehydrogenase enzyme activity in tissue sections.
• Microbiological Testing: Applied in assays to detect microbial growth and metabolic function.
• Biochemical Research: Serves as an electron acceptor in studies of mitochondrial function and redox biology.
• Diagnostic Kit Manufacturing: A key component in the formulation of kits for clinical and research diagnostics.
• Pharmaceutical Development: Utilized in stability testing and formulation studies to assess product-cell interactions.

Basic Information

Product Name	p-Anisyl Blue Tetrazolium Chloride
CAS No.	127615-61-6
Molecular Formula	C24H22ClN5O3
Molecular Weight	463.91 g/mol
Synonyms	p-Anisyl Blue Tetrazolium Chloride; 4-Methoxyphenyl Blue Tetrazolium Chloride; 2,2'-(4-Methoxyphenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-biphenylene)ditetrazolium Chloride; Anisyl Blue Tetrazolium Chloride; p-ABT; BT Anisyl Blue; Tetrazolium Blue p-Anisyl Derivative
EINECS	Contact for details

Quality Control

Our p-Anisyl Blue Tetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for sensitive research applications. Certificates of Analysis (COA) detailing specifications such as purity, absorbance, and solubility are provided to guarantee traceability and batch-to-batch reliability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Absorbance (λ max)	Conforms
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.