Description

7-Methoxycoumarin-3-Carboxylyl-Prolyl-Leucyl-Glycyl-Prolyl-Lysyl-2,4-Dinitrophenyl is a specialized, high-purity synthetic peptide derivative designed for advanced biochemical research and development. This compound is valued for its role as a sophisticated fluorogenic substrate or a key intermediate in the synthesis of enzyme inhibitors and diagnostic probes. It is primarily utilized by research institutions and pharmaceutical companies focused on protease activity studies, high-throughput screening assays, and the development of novel therapeutic agents. The product is supplied with a commitment to stringent quality control, ensuring reliability for sensitive experimental applications.

Application

• Protease & Enzyme Research: Acts as a fluorogenic or chromogenic substrate for studying the kinetics and inhibition of specific proteases, including matrix metalloproteinases (MMPs) and other serine or cysteine proteases.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify and characterize potential enzyme inhibitors in pharmaceutical R&D.
• Biochemical Assay Development: Serves as a critical component in developing sensitive and specific diagnostic or research assays for enzymatic activity.
• Peptide Chemistry & Conjugation: Used as a building block or a labeled intermediate in the synthesis of more complex peptide conjugates for targeted delivery or imaging.
• Academic & Institutional Research: Supports fundamental biochemical and cell biology studies investigating proteolytic pathways and signal transduction.

Basic Information

Item	Detail
Product Name	7-Methoxycoumarin-3-Carboxylyl-Prolyl-Leucyl-Glycyl-Prolyl-Lysyl-2,4-Dinitrophenyl
CAS No.	127376-94-7
Molecular Formula	C48H60N10O16
Molecular Weight	1009.06 g/mol
Synonyms	Mca-Pro-Leu-Gly-Pro-Lys(Dnp); Mca-PLGPK(Dnp); Fluorogenic Substrate for MMPs; 7-Methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys(Dnp)-OH; MMP Substrate III; CAS 127376-94-7; Dnp-PLGPK-Mca; (7-Methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Pro-Lys(2,4-dinitrophenyl)
EINECS	Contact for details

Quality Control

Every batch of 7-Methoxycoumarin-3-Carboxylyl-Prolyl-Leucyl-Glycyl-Prolyl-Lysyl-2,4-Dinitrophenyl is manufactured and analyzed under strict quality management protocols to ensure it meets the high-purity standards required for research applications. Our quality assurance includes comprehensive analytical testing via HPLC, MS, and NMR to verify identity, purity, and consistency. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting all critical specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccation and storage under inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area Percent)
Assay (HPLC)	≥ 98% (on dried basis)
Water Content (KF)	≤ 3.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.