Description

5-(3-(2,2,6,6-Tetramethyl-1-Oxy-Piperidine-4-Carboxamido)Prop-1-Enyl)-2'-Deoxycytidine 5'-Triphosphate is a specialized, spin-labeled nucleotide triphosphate analog designed for advanced biophysical and biochemical research. This compound is critical for studying nucleic acid structure, dynamics, and protein-nucleic acid interactions using techniques like Electron Paramagnetic Resonance (EPR) spectroscopy. It is primarily utilized by research scientists and pharmaceutical developers in the fields of structural biology, enzymology, and next-generation nucleic acid probe development.

Application

• EPR/ESR Spectroscopy Studies: Acts as a site-specific spin label for probing the conformation, flexibility, and local environment of DNA and RNA.
• Nucleic Acid Dynamics Research: Used to investigate real-time structural changes, folding, and macromolecular interactions within nucleic acid systems.
• Enzyme Mechanism Analysis: Serves as a substrate analog to study the kinetics and catalytic mechanisms of DNA polymerases, kinases, and other nucleic acid-processing enzymes.
• Structural Biology: Aids in determining three-dimensional structures and mapping binding sites in protein-DNA/RNA complexes.
• Biophysical Probe Development: Incorporated into oligonucleotides to create sensitive probes for diagnostic assays and sensor technologies.
• Drug Discovery Screening: Used in assays to identify and characterize compounds that interact with specific DNA or RNA targets.

Basic Information

Product Name	5-(3-(2,2,6,6-Tetramethyl-1-Oxy-Piperidine-4-Carboxamido)Prop-1-Enyl)-2'-Deoxycytidine 5'-Triphosphate
CAS No.	127302-77-6
Molecular Formula	C23H35N6O15P3
Molecular Weight	752.49 g/mol
Synonyms	Spin-labeled dCTP; TEMPO-dCTP; 2'-Deoxycytidine-5'-triphosphate, 5-[3-[[(2,2,6,6-tetramethyl-1-oxyl-4-piperidinyl)carbonyl]amino]-1-propen-1-yl]-; SL-dCTP; Nitroxide-labeled dCTP; dCTP-TEMPO; Proxyl-dCTP; EPR-active dCTP
EINECS	Contact for details

Quality Control

Every batch of our spin-labeled nucleotide is manufactured and analyzed under strict quality protocols to ensure the highest standards of purity and performance for research applications. We provide comprehensive Certificates of Analysis (COA) detailing purity (HPLC), identity (NMR, MS), and specific activity, supporting traceability and reproducibility in your experiments. Our quality system is designed to meet the rigorous demands of academic and pharmaceutical R&D.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (UV)	≥ 90% (as triphosphate)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in aqueous buffer, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.