Description

γ-Tetrazole-Methotrexate is a chemically modified derivative of the established antifolate agent methotrexate, incorporating a tetrazole moiety. This structural modification is primarily of interest in advanced pharmaceutical research for the development of novel therapeutic agents and diagnostic tools. It serves as a critical intermediate or reference standard for researchers in oncology and immunology, particularly those investigating targeted drug delivery and prodrug strategies. The compound's unique properties make it valuable for specialized applications in drug discovery and development.

Application

• Pharmaceutical Intermediate: Serves as a key building block in the synthesis of novel methotrexate analogs and conjugates for enhanced therapeutic profiles.
• Research Chemical: Used as a reference standard in analytical method development and validation for quality control laboratories.
• Prodrug Development: Investigated for its potential in creating targeted prodrugs that release methotrexate under specific physiological conditions.
• Bioconjugation Studies: The tetrazole group can be utilized in bioorthogonal chemistry for labeling antibodies or other biomolecules with cytotoxic agents.
• Enzyme Inhibition Research: Employed in biochemical assays to study the inhibition kinetics of dihydrofolate reductase (DHFR) and other folate pathway enzymes.
• Academic & Institutional Research: Supports fundamental research in medicinal chemistry and pharmacology at universities and R&D institutes.

Basic Information

Product Name	γ-Tetrazole-Methotrexate
CAS No.	127105-48-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5-Methyltetrazolyl Methotrexate; MTX-Tetrazole; Methotrexate Tetrazole Derivative; N-[(2,4-Diaminopteridin-6-yl)methyl]-N-methyl-4-[[(2-amino-4-oxo-1,4-dihydro-6-pteridinyl)methyl]amino]benzoyl]-L-glutamic Acid Tetrazole Analog; Tetrazole-MTX; γ-Tetrazole Methotrexate
EINECS	Contact for details

Quality Control

Our γ-Tetrazole-Methotrexate is produced under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for research and development purposes. Each batch undergoes rigorous analytical testing, which may include HPLC for purity assessment, NMR and MS for structural confirmation, and residual solvent analysis. A comprehensive Certificate of Analysis (COA) detailing all test results and specifications is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent degradation. For long-term storage, consider conditions under an inert atmosphere.

Specification

Item	Specification
Appearance	Off-white to yellow solid powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	≤ 5.0% total
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.