Description

3-Indoxyl-β-D-Galactopyranoside is a specialized chromogenic substrate widely used in molecular biology and diagnostic applications. Its core value lies in its enzymatic cleavage by β-galactosidase, producing an insoluble blue indigo dye that serves as a highly sensitive and visually detectable reporter. This compound is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and in-vitro diagnostic (IVD) sectors. It is a critical reagent for applications such as gene expression assays, bacterial colony screening, and histological staining.

Application

• Molecular Biology Research: As a substrate for β-galactosidase in reporter gene assays (e.g., lacZ gene) for monitoring gene expression and promoter activity.
• Microbiology & Diagnostics: For the detection and identification of β-galactosidase-producing microorganisms, such as E. coli, in culture media and diagnostic kits.
• Histology & Cell Biology: Used in enzymatic histochemistry and cytochemistry for in-situ localization of β-galactosidase activity in tissue sections and cell cultures.
• High-Throughput Screening (HTS): Employed in drug discovery and biochemical assays to screen for enzyme inhibitors or activators.
• IVD Reagent Manufacturing: A key component in the formulation of chromogenic substrates for clinical diagnostic tests and automated analyzer systems.
• Plant Biology: Utilized in transgenic plant research to visualize and quantify reporter gene expression.

Basic Information

Product Name	3-Indoxyl-β-D-Galactopyranoside
CAS No.	126787-65-3
Molecular Formula	C20H27NO11
Molecular Weight	457.43 g/mol
Synonyms	X-Gal; 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside (Note: This is the bromo-chloro derivative, a closely related and more common substrate); 3-Indolyl-β-D-galactopyranoside; Indoxyl-β-D-galactoside; β-Galactosidase substrate; Galactopyranosyl indoxyl; IBDG
EINECS	Contact for details

Quality Control

Our 3-Indoxyl-β-D-Galactopyranoside is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and spectroscopic identification. We provide comprehensive Certificates of Analysis (COA) detailing all specifications, ensuring the product meets the stringent requirements for research and diagnostic applications. Our quality commitment supports compliance with relevant industry standards for biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term stability, consider storage under inert atmosphere or with desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Specific Rotation	Contact for details
Heavy Metals	< 10 ppm
Biological Activity	Suitable for β-galactosidase assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.