Description

Methyl 3-Methoxy-2-(4-(3-Trifluoro-3-Diazirinyl)Benzoyloxyphenyl)Propenoate is a specialized, high-purity photoaffinity labeling reagent featuring a trifluoromethyl diazirine group. This compound is engineered for advanced cross-linking studies, enabling the covalent capture of transient or weak biomolecular interactions upon UV light activation. It is an essential tool for researchers in pharmaceutical discovery, chemical biology, and proteomics who require precise and irreversible target identification.

Application

• Photoaffinity Labeling (PAL): A critical reagent for mapping drug-protein and protein-protein interaction sites in drug discovery and structural biology.
• Chemical Proteomics: Used in activity-based protein profiling (ABPP) to identify and characterize enzyme active sites and binding pockets.
• Bioconjugation & Probe Development: Serves as a key intermediate for synthesizing bioactive probes with photo-crosslinking capabilities.
• Target Identification & Validation: Enables the covalent tagging of molecular targets for downstream analysis via mass spectrometry or fluorescence.
• Materials Science Research: Applied in surface modification and polymer chemistry to create light-responsive, functionalized materials.

Basic Information

Product Name	Methyl 3-Methoxy-2-(4-(3-Trifluoro-3-Diazirinyl)Benzoyloxyphenyl)Propenoate
CAS No.	125808-18-6
Molecular Formula	C20H15F3N2O5
Molecular Weight	420.34 g/mol
Synonyms	3-(Trifluoromethyl)-3-phenyldiazirine-3'-carboxylic acid, 4'-(2-methoxycarbonyl)ethenylphenyl ester; Methyl (E)-3-methoxy-2-[4-[[3-(trifluoromethyl)-3H-diazirin-3-yl]carbonyl]oxy]phenyl]propenoate; TFD Methyl Ester Photoaffinity Label; 3-Trifluoromethyl-3-phenyldiazirine derivative; Photo-crosslinking reagent; PAL reagent; Benzophenone analog photoaffinity probe.
EINECS	Contact for details

Quality Control

Every batch of our photoaffinity labeling reagents is manufactured under strict quality protocols to ensure exceptional purity and performance consistency, critical for sensitive research applications. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity (HPLC), identity (NMR, IR), and stability data. Our quality system is designed to support GMP/GLP-compliant research and development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dedicated freezer. This compound is strictly light-sensitive and should be handled under subdued light. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.