Description

4-Nitrophenyl 2-Acetamido-3-o-(2-Acetamido-2-Deoxy-B-D-Glucopyranosyl)-2-Deoxy-A-D-Galactopyranoside is a specialized synthetic glycoside derivative, primarily utilized as a chromogenic substrate in enzymatic assays. Its core value lies in enabling precise biochemical research and diagnostic development by serving as a cleavable substrate for specific glycosidases, releasing a quantifiable colored product. This compound is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors who require high-purity biochemical tools.

Application

• Enzymatic Substrate for Glycosidases: Acts as a chromogenic substrate for the detection and kinetic study of specific enzymes like β-N-acetylhexosaminidases.
• Biochemical Research: Used in fundamental research to investigate carbohydrate metabolism, enzyme mechanisms, and glycobiology pathways.
• Diagnostic Kit Development: A key component in the formulation of diagnostic assays and kits for detecting enzyme activity related to certain genetic disorders (e.g., Tay-Sachs disease, Sandhoff disease).
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential inhibitors or activators of target glycosidase enzymes for drug discovery.
• Quality Control in Biomanufacturing: Used to assay the activity of enzyme preparations used in therapeutic production.

Basic Information

Item	Detail
Product Name	4-Nitrophenyl 2-Acetamido-3-o-(2-Acetamido-2-Deoxy-B-D-Glucopyranosyl)-2-Deoxy-A-D-Galactopyranoside
CAS No.	125455-64-3
Molecular Formula	C26H37N3O15
Molecular Weight	631.59 g/mol
Synonyms	p-Nitrophenyl N,N'-diacetyl-β-chitobioside; 4-Nitrophenyl N,N'-diacetyl-β-chitobioside; PNP-(GlcNAc)2; 4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(2-acetamido-2-deoxy-β-D-glucopyranosyl)-α-D-galactopyranoside; pNP-β-D-GlcNAc-(1→4)-α-D-GalNAc; Chromogenic substrate for β-N-acetylhexosaminidase; N,N'-Diacetylchitobioside p-nitrophenyl
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl 2-Acetamido-3-o-(2-Acetamido-2-Deoxy-B-D-Glucopyranosyl)-2-Deoxy-A-D-Galactopyranoside is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the exacting standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric humidity. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.