Description

Gly-Arg p-Nitroanilide Dihydrochloride is a specialized chromogenic peptide substrate used in enzymatic assays. This compound is critical for the precise measurement of trypsin-like protease activity, offering high sensitivity and specificity. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries for enzyme kinetics studies and biochemical research.

Application

• Protease Activity Assays: As a specific chromogenic substrate for the determination of trypsin, thrombin, and other serine protease activities.
• Biochemical Research: Used in enzyme kinetics studies to investigate catalytic mechanisms and inhibitor screening.
• Pharmaceutical Development: Employed in quality control and potency testing of enzyme-based therapeutics and diagnostic reagents.
• Diagnostic Manufacturing: A key component in the formulation of clinical diagnostic kits for measuring proteolytic activity in biological samples.
• Academic Research: Utilized in university and institutional labs for fundamental studies in biochemistry and molecular biology.

Basic Information

Item	Details
Product Name	Gly-Arg p-Nitroanilide Dihydrochloride
CAS No.	125455-61-0
Molecular Formula	C14H22Cl2N8O5
Molecular Weight	453.28 g/mol
Synonyms	Glycyl-L-arginine 4-nitroanilide dihydrochloride; H-Gly-Arg-pNA·2HCl; GARPNA; L-Glycyl-L-arginine 4-nitroanilide dihydrochloride; Gly-Arg-pNA·2HCl; Gly-Arg-4-NA·2HCl; Nα-Glycyl-Nω-nitro-L-arginine 4-nitroanilide dihydrochloride; Trypsin Substrate
EINECS	Contact for details

Quality Control

Our Gly-Arg p-Nitroanilide Dihydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR spectroscopy, and verification of physical properties. Certificates of Analysis (COA) documenting all test results are provided to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive) and should be handled in a low-humidity environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Clear, colorless solution in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.