Description

'Caged GTP CAS NO 125455-57-4 is a chemically modified, photolabile derivative of guanosine triphosphate (GTP) designed for precise, light-triggered release in biological research. This compound is critical for studying real-time dynamics of GTP-binding proteins (G-proteins), small GTPases, and other nucleotide-dependent processes with high spatial and temporal control. It is an essential tool for researchers in cell biology, neurobiology, and signal transduction who require a non-invasive method to activate specific pathways on demand.

Application

• Real-time Kinetics Studies: Uncaging GTP with UV light to initiate and monitor G-protein and small GTPase (e.g., Ras, Rho, Rab) activity in live cells or reconstituted systems.
• Neurobiological Research: Investigating the role of GTP in synaptic vesicle recycling, neurotransmitter release, and neuronal signaling cascades with millisecond precision.
• Cell Physiology & Development: Spatially controlled activation of GTPases to study cytoskeletal dynamics, cell motility, division, and differentiation.
• High-Resolution Imaging: Used in conjunction with fluorescence microscopy (e.g., FRET, TIRF) to correlate localized GTP release with downstream cellular events.
• Enzyme Mechanism Analysis: A substrate for studying the kinetics and regulation of GTPases, nucleotidyltransferases, and polymerases in vitro.
• Drug Discovery Screening: Facilitating assays for identifying modulators of GTP-binding protein function and related therapeutic targets.

Basic Information

Product Name	'Caged GTP
CAS No.	125455-57-4
Molecular Formula	C₁₇H₂₃N₆O₁₇P₃ (typically as a salt, e.g., lithium or tetraethylammonium)
Molecular Weight	Contact for details (varies by salt form)
Synonyms	P³-1-(2-Nitrophenyl)ethyl Guanosine-5'-Triphosphate; NPE-caged GTP; 2-Nitrobenzyl-caged GTP; Guanosine-5'-triphosphate, P³-1-(2-nitrophenyl)ethyl ester; caged guanosine triphosphate; Photolabile GTP; 2-NPE-GTP; Light-activatable GTP
EINECS	Contact for details

Quality Control

Our 'Caged GTP is produced and handled under strict quality protocols to ensure batch-to-batch consistency and optimal performance in sensitive biological assays. Each lot is characterized to confirm identity, purity, and photolytic efficiency. A comprehensive Certificate of Analysis (COA) is provided, detailing specifications such as HPLC purity, extinction coefficient, and uncaging efficiency. We support research requiring GMP-grade materials and can accommodate custom purity requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at or below -20°C in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Aliquoting is recommended to avoid repeated freeze-thaw cycles and exposure to ambient conditions. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Anhydrous basis)
Water Content (KF)	≤ 3.0%
Extinction Coefficient (ε)	Provided per batch
Photolytic Release Efficiency	≥ 80% (upon UV irradiation, typical)
Biological Activity	Functional in standard GTPase activation assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.