Description

5-Bromo-4-Chloro-3-Indolyl α-D-Mannopyranoside is a specialized chromogenic substrate used for the detection and quantification of α-mannosidase enzyme activity. This compound matters for its high sensitivity and specificity in biochemical assays, enabling precise enzymatic analysis in research and diagnostic applications. It is primarily needed by biotechnology, pharmaceutical, and diagnostic kit manufacturers for applications in enzyme kinetics, cell biology research, and the development of diagnostic tools.

Application

• Enzyme Histochemistry & Cytochemistry: Used as a substrate for the localization of α-mannosidase activity in tissue sections and cell cultures, producing an insoluble blue indigo dye upon cleavage.
• Biochemical Research & Assay Development: Essential for in vitro enzymatic studies, kinetic analysis, and high-throughput screening (HTS) to identify enzyme inhibitors or activators.
• Diagnostic Kit Manufacturing: Incorporated into commercial diagnostic kits and reagents for the detection of lysosomal storage disorders, such as α-mannosidosis, and other related metabolic diseases.
• Microbiology & Cell Biology: Employed in studies of glycosidase activity in microorganisms and for tracking specific enzymatic pathways within eukaryotic cells.
• Quality Control in Bioprocessing: Used to monitor and ensure the consistency and activity of α-mannosidase enzymes produced for therapeutic or industrial use.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl α-D-Mannopyranoside
CAS No.	125229-64-3
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	X-α-D-Mannopyranoside; 5-Bromo-4-chloro-3-indolyl-α-D-mannopyranoside; BCIP-α-Mannoside; α-Mannosidase Substrate; Indoxyl-α-D-mannopyranoside (5-Br-4-Cl derivative); 3-Indolyl-α-D-mannopyranoside, 5-bromo-4-chloro-; X-α-Man
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl α-D-Mannopyranoside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; sparingly soluble in water
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.