Description

5'-O-(4,4'-Dimethoxytrityl)Thymidine-3'-O-(2,4-Dinitrophenyl) Succinate is a critical, high-purity phosphoramidite building block used in the automated synthesis of oligonucleotides. This compound matters because it provides the protected thymidine monomer necessary for constructing DNA sequences with high coupling efficiency and minimal side reactions. It is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries who require reliable, high-quality raw materials for antisense therapeutics, PCR primers, probes, and other genomic applications.

Application

• Oligonucleotide Synthesis: Serves as a key protected thymidine (dT) monomer in solid-phase phosphoramidite chemistry for automated DNA synthesizers.
• Antisense & siRNA Therapeutics: Used in the production of therapeutic oligonucleotides targeting specific genes for the treatment of various diseases.
• Diagnostic Probe Manufacture: Essential for creating labeled DNA probes used in hybridization assays, microarrays, and next-generation sequencing (NGS).
• PCR Primer Production: Incorporated into the synthesis of precise primers for polymerase chain reaction (PCR) and quantitative PCR (qPCR).
• Research & Development: A vital reagent in academic and industrial R&D labs for studying gene function, regulation, and for developing novel genetic tools.
• Synthetic Biology: Used in the construction of synthetic genes, genetic circuits, and engineered biological systems.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)Thymidine-3'-O-(2,4-Dinitrophenyl) Succinate
CAS No.	125078-79-7
Molecular Formula	C43H39N5O13
Molecular Weight	857.80 g/mol
Synonyms	DMT-dT-DNP Succinate; 5'-O-DMT-Thymidine-3'-O-(2,4-Dinitrophenyl) Succinate; Thymidine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-3'-O-[3-[(2,4-dinitrophenyl)amino]-3-oxopropanoyl]-; DMT-Thy(dnp)succinate; N3-(2,4-Dinitrophenyl)-5'-O-(4,4'-dimethoxytrityl)thymidine-3'-succinate; DNP-Succinyl-DMT-dT; DNA Synthesis Reagent dT(DNP)CE Phosphoramidite precursor
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)Thymidine-3'-O-(2,4-Dinitrophenyl) Succinate is manufactured under strict quality systems to ensure it meets the exacting standards required for oligonucleotide synthesis. Every batch is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee consistency, reliability, and traceability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions. For long-term storage, consider storing under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.