Description

(n-(7-(Nitrobenz-2-Oxa-1,3-Diazol-4-Yl))-7-Amino-3Alpha,12Alpha-Dihydroxycholan-24-Oyl)-2-Aminoethanesulfonate is a specialized fluorescent bile acid derivative, designed for advanced research and diagnostic applications. Its core value lies in its unique structure, which combines a fluorescent NBD (nitrobenzoxadiazole) reporter group with a cholic acid backbone, enabling sensitive detection and tracking in complex biological systems. This compound is essential for researchers and developers in the fields of molecular biology, hepatology, and diagnostic assay development, particularly for studying bile acid transport, metabolism, and receptor interactions.

Application

• Fluorescent Probe for Bile Acid Transporters: Used to visualize and quantify the activity of key transporters like NTCP, ASBT, and BSEP in cellular and in vitro models.
• Biomarker Research: Serves as a critical tool in the study of liver function, cholestasis, and metabolic disorders related to bile acid pathways.
• High-Throughput Screening (HTS): Employed in drug discovery campaigns to identify modulators of bile acid signaling and transport for therapeutic development.
• Diagnostic Kit Development: Acts as a key component in the formulation of sensitive ELISA or fluorescence-based diagnostic kits for clinical analysis.
• Cellular Imaging Studies: Enables real-time, high-resolution imaging of bile acid uptake and intracellular trafficking in live cells.
• Receptor Binding Assays: Utilized in competitive binding studies to investigate interactions with nuclear receptors such as FXR and TGR5.

Basic Information

Product Name	(n-(7-(Nitrobenz-2-Oxa-1,3-Diazol-4-Yl))-7-Amino-3Alpha,12Alpha-Dihydroxycholan-24-Oyl)-2-Aminoethanesulfonate
CAS No.	124882-57-1
Molecular Formula	C33H45N5O9S
Molecular Weight	687.81 g/mol
Synonyms	NBD-Tauro-CA; NBD-Taurocholic Acid; NBD-TCA; 7-Nitrobenz-2-oxa-1,3-diazol-4-yl-taurocholic Acid; Tauro-NBD-cholic Acid; Fluorescent Taurocholic Acid; NBD-CT; NBD-Labeled Cholic Acid Taurine Conjugate
EINECS	Contact for details

Quality Control

Our (n-(7-(Nitrobenz-2-Oxa-1,3-Diazol-4-Yl))-7-Amino-3Alpha,12Alpha-Dihydroxycholan-24-Oyl)-2-Aminoethanesulfonate is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical research. Each lot undergoes comprehensive analytical testing, including HPLC purity analysis, mass spectrometry (MS) confirmation, and fluorescence spectroscopy. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting identity, purity, and specific performance characteristics.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Fluorescence Emission	~535 nm (in methanol)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, methanol; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.