Description

Restriction Endonuclease Kpn 2I is a high-purity, site-specific endonuclease enzyme used for precise DNA cleavage in molecular biology research and biotechnological applications. Its primary value lies in providing reliable and reproducible digestion of DNA at its specific recognition sequence, which is critical for genetic engineering, cloning, and genomic analysis workflows. This product is essential for research laboratories, diagnostic kit manufacturers, and biotechnology companies engaged in genetic research, synthetic biology, and pharmaceutical development.

Application

• Molecular Cloning: Essential for the precise insertion of DNA fragments into plasmid vectors.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: Used in genetic fingerprinting and mutation detection.
• Genomic DNA Mapping: Facilitates the construction of physical maps of genomes.
• Recombinant DNA Technology: A key tool for constructing recombinant DNA molecules in synthetic biology.
• Diagnostic Assay Development: Incorporated into kits for the detection of specific genetic sequences or mutations.
• DNA Sequencing Library Preparation: Used in next-generation sequencing workflows to fragment or prepare DNA samples.

Basic Information

Product Name	Restriction Endonuclease Kpn 2I
CAS No.	124834-24-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Kpn2I; Endodeoxyribonuclease Kpn2I; Kpn2I Restriction Enzyme; Type II Restriction Enzyme Kpn2I; Restriction Enzyme Kpn2I; Kpn 2I; EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Kpn 2I is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and optimal activity. Each lot is rigorously tested for specific activity, purity (free from non-specific nucleases and phosphatases), and stability. A comprehensive Certificate of Analysis (COA) detailing activity units, purity assays, and storage buffer composition is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in the supplied storage buffer. Avoid repeated freeze-thaw cycles. Keep the enzyme on ice or in a chilled block during use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Exonuclease Activity	Not detectable
Endonuclease Activity	Not detectable
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.