Description

5-Bromoindoxyl-β-Cellobioside is a specialized chromogenic substrate used in enzymatic assays. This compound matters for its high specificity in detecting and quantifying β-glucosidase and cellulase enzyme activity, which is critical for research and quality control in various industries. It is primarily needed by biochemical researchers, diagnostic kit manufacturers, and industrial enzyme producers working in life sciences, biofuels, and food processing.

Application

• Biochemical Research: As a specific substrate for studying the kinetics and inhibition of β-glucosidase and cellulase enzymes.
• Diagnostic Assays: Used in the development of chromogenic tests for enzyme-linked immunosorbent assays (ELISA) and other clinical diagnostics.
• Enzyme Activity Screening: For high-throughput screening of microbial or fungal cultures for cellulolytic activity in biofuel and biorefinery applications.
• Quality Control in Biotechnology: To assay the purity and specific activity of commercial enzyme preparations.
• Histochemistry & Cell Biology: As a staining agent for the localization of β-glucosidase activity in tissue sections and cell cultures.
• Food & Beverage Industry: For process monitoring and quality assurance in applications involving enzymatic breakdown of cellulose.

Basic Information

Product Name	5-Bromoindoxyl-β-Cellobioside
CAS No.	124613-91-8
Molecular Formula	C20H24BrNO11
Molecular Weight	534.31 g/mol
Synonyms	5-Bromo-3-indolyl β-D-cellobioside; 5-Bromoindoxyl β-cellobioside; 5-Br-Indoxyl-β-Cellobioside; 5-Bromo-3-indoxyl-β-D-cellobioside; BIC; 5-Bromo-1H-indol-3-yl β-D-glucopyranosyl-(1→4)-β-D-glucopyranoside; Chromogenic β-glucosidase substrate; Cellobioside, 5-bromoindoxyl
EINECS	Contact for details

Quality Control

Our 5-Bromoindoxyl-β-Cellobioside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a cool, dry place. The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content	≤ 5.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.