Description

4-Methylumbelliferyl 2-Acetamido-2-Deoxy-α-D-Galactopyranoside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of α-N-acetylgalactosaminidase (α-NAGA) enzyme activity. This compound matters because it enables precise biochemical research and diagnostic applications, offering high specificity and a strong fluorescent signal upon enzymatic cleavage. It is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors for enzyme kinetics studies, lysosomal storage disorder screening, and high-throughput assay development.

Application

• Enzymatic Assay Substrate: Primary fluorogenic substrate for the detection and kinetic analysis of α-N-acetylgalactosaminidase (α-NAGA) activity.
• Diagnostic Reagent Manufacturing: Key component in the production of diagnostic kits for lysosomal storage disorders, such as Schindler disease and Kanzaki disease.
• Biochemical Research: Used in research laboratories for studying glycosidase enzyme mechanisms, inhibitor screening, and pathway analysis.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify potential therapeutic modulators of α-NAGA enzyme function.
• Quality Control in Bioprocessing: Acts as a specific assay reagent to monitor enzyme purity and activity during the production of recombinant proteins or enzyme therapies.
• Cell Biology Studies: Utilized in cellular imaging and flow cytometry to probe glycosidase activity within live or fixed cells.

Basic Information

Product Name	4-Methylumbelliferyl 2-Acetamido-2-Deoxy-α-D-Galactopyranoside
CAS No.	124223-99-0
Molecular Formula	C₁₈H₂₁NO₉
Molecular Weight	395.36 g/mol
Synonyms	4-MU-α-GalNAc; 4-Methylumbelliferyl α-N-acetyl-D-galactosaminide; 4-Methylumbelliferyl N-acetyl-α-D-galactosaminide; α-NAGA Substrate; α-N-Acetylgalactosaminidase Fluorogenic Substrate; 4-MU-α-D-GalNAc; 2-Acetamido-2-deoxy-α-D-galactopyranosyl 4-methylumbelliferone; 4-Methylumbelliferyl 2-acetamido-2-deoxy-α-D-galactopyranoside
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl 2-Acetamido-2-Deoxy-α-D-Galactopyranoside is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and spectroscopic methods for identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Specific Optical Rotation	Contact for details
Fluorescence Background	Low (specified in COA)
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.