Description

4-Methylumbelliferyl2-Acetamido-3,4,6-Tri-o-Acetyl-2-Deoxy-B-D-Glucopyranoside is a specialized fluorogenic substrate used for the detection and quantification of specific glycosidase enzyme activity. This compound matters for researchers and quality control labs requiring high-sensitivity, precise enzymatic assays. It is primarily needed by the biotechnology, pharmaceutical, and diagnostic industries for applications in enzyme kinetics, high-throughput screening, and biochemical research.

Application

• Enzymatic Substrate for Glycosidases: Specifically used as a fluorogenic substrate for N-acetyl-β-D-glucosaminidase and related enzymes.
• Biochemical Research & Assay Development: Enables the study of enzyme mechanisms, inhibition, and kinetics through fluorescence-based detection.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for lysosomal storage disorders and other enzyme-linked conditions.
• High-Throughput Screening (HTS): Utilized in pharmaceutical discovery to identify potential enzyme inhibitors or activators in drug candidate libraries.
• Microbiology & Cell Biology: Assists in the study of bacterial or cellular glycosidase activity as a reporter system.
• Quality Control in Bioprocessing: Monitors enzyme activity and purity during the production of therapeutic proteins and biologics.

Basic Information

Product Name	4-Methylumbelliferyl2-Acetamido-3,4,6-Tri-o-Acetyl-2-Deoxy-B-D-Glucopyranoside
CAS No.	124167-45-9
Molecular Formula	C24H27NO13
Molecular Weight	537.47 g/mol
Synonyms	4-MU-β-GlcNAc-3,4,6-triacetate; 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide triacetate; 4-Methylumbelliferyl 2-acetamido-2-deoxy-β-D-glucopyranoside-3,4,6-triacetate; 4-MU-β-GlcNAc-3,4,6-TriAc; Fluorogenic NAG substrate; 4-MU-GlcNAc(TriAc); 2-Acetamido-2-deoxy-β-D-glucopyranoside 3,4,6-triacetate 4-methylumbelliferyl ester
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Every batch is characterized to confirm identity, purity, and performance as a fluorogenic substrate. Certificates of Analysis (COA) are available upon request, detailing specifications such as HPLC purity, fluorescence performance, and absence of interfering impurities.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light may affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Fluorescence Performance	Conforms to reference standard
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.