Description

4-Methylumbelliferyl 3,4,6-Tri-o-Acetyl-2-Azido-2-Deoxy-α-D-Galactopyranoside is a specialized fluorogenic glycosidase substrate, crucial for the sensitive detection and kinetic analysis of specific enzymatic activity. Its core value lies in its role as a key biochemical tool for research and diagnostic assay development, where the release of the highly fluorescent 4-methylumbelliferone upon enzymatic cleavage provides a quantifiable signal. This compound is primarily needed by researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors for studying glycosidase enzymes and developing high-throughput screening assays.

Application

• Enzymology Research: As a specific fluorogenic substrate for α-N-acetylgalactosaminidase and related glycosidases to study enzyme kinetics, inhibition, and mechanism of action.
• Diagnostic Assay Development: Used in the design of clinical diagnostic kits for the detection of lysosomal storage disorders, such as Schindler disease, which are linked to deficiencies in specific glycosidases.
• High-Throughput Screening (HTS): Employed in pharmaceutical and biotechnology research to screen compound libraries for potential inhibitors or activators of target glycosidase enzymes.
• Cell Biology & Histochemistry: Utilized in in-situ enzymatic activity assays within cells or tissue sections to localize and quantify glycosidase expression.
• Biomarker Discovery: Aids in research focused on identifying and validating glycosidase activity as a potential biomarker for various diseases, including cancer.
• Quality Control in Biologics: Can be applied in the characterization and quality control of enzyme-based therapeutics to ensure specific activity and purity.

Basic Information

Product Name	4-Methylumbelliferyl 3,4,6-Tri-o-Acetyl-2-Azido-2-Deoxy-α-D-Galactopyranoside
CAS No.	124167-43-7
Molecular Formula	C₂₂H₂₄N₄O₁₁
Molecular Weight	520.45 g/mol
Synonyms	4-MU-α-GalNAc(Ac)₃-Az; 4-Methylumbelliferyl 2-Azido-2-deoxy-3,4,6-tri-O-acetyl-α-D-galactopyranoside; 2-Azido-2-deoxy-3,4,6-tri-O-acetyl-α-D-galactopyranosyl 4-methylumbelliferone; 4-MU-α-D-GalNAc(Ac)₃-N₃; α-4-MU-GalNAc(Ac)₃-Azide; Fluorogenic α-N-acetylgalactosaminidase substrate; Galactosidase substrate, 4-methylumbelliferyl deriv.
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl 3,4,6-Tri-o-Acetyl-2-Azido-2-Deoxy-α-D-Galactopyranoside is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and structural confirmation via NMR and mass spectrometry. Certificates of Analysis (COA) detailing all specifications and test results are provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.