Description

2-Chloro-4-Nitrophenyl-β-D-Galactopyranoside is a specialized chromogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of β-galactosidase activity, a key enzyme in molecular biology and diagnostic applications. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies for applications in gene expression studies, reporter assays, and clinical diagnostics.

Application

• Enzymatic Substrate for β-Galactosidase: Used as a chromogenic reagent in biochemical assays to measure β-galactosidase enzyme activity, commonly employed in molecular biology research.
• Reporter Gene Assays: Essential in lacZ reporter gene systems for monitoring gene expression and promoter activity in cell cultures and transgenic models.
• Diagnostic Kits: A key component in the formulation of diagnostic kits for bacterial detection (e.g., E. coli) and certain genetic disorders involving lysosomal enzyme deficiencies.
• High-Throughput Screening (HTS): Utilized in pharmaceutical and biotechnology labs for drug discovery screens targeting β-galactosidase or related pathways.
• Histochemistry & Cell Staining: Applied in histological techniques for in situ localization of β-galactosidase activity in tissue samples.
• Quality Control in Biotechnology: Used to assess the activity and consistency of enzyme preparations and recombinant protein products.

Basic Information

Product Name	2-Chloro-4-Nitrophenyl-β-D-Galactopyranoside
CAS No.	123706-60-5
Molecular Formula	C12H14ClNO8
Molecular Weight	335.69 g/mol
Synonyms	2-Chloro-4-nitrophenyl β-D-galactopyranoside; CNPG; 2-Chloro-4-nitrophenyl-β-D-galactoside; β-Galactosidase Substrate (2-Chloro-4-nitrophenyl); 4-Nitro-2-chlorophenyl β-D-galactopyranoside; Galactopyranoside, 2-chloro-4-nitrophenyl, β-D-; 2-Chloro-4-nitrophenyl-1-thio-β-D-galactopyranoside (related thio analog); o-Nitrophenyl-β-D-galactopyranoside (ONPG) analog
EINECS	Contact for details

Quality Control

Our 2-Chloro-4-Nitrophenyl-β-D-Galactopyranoside is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.