Description

2-o-(p-Nitrophenyl)-α-D-n-Acetylneuraminic Acid, Sodium Salt, X Hydrate is a chemically modified derivative of N-acetylneuraminic acid (Neu5Ac), a fundamental sialic acid. This compound serves as a critical chromogenic substrate for the detection and kinetic study of neuraminidase (sialidase) enzymes, which are pivotal in viral infection, bacterial pathogenesis, and cellular signaling pathways. It is an essential research tool for virologists, microbiologists, and biochemists in pharmaceutical R&D, diagnostic development, and academic research focused on influenza, other pathogens, and glycobiology.

Application

• Neuraminidase (Sialidase) Activity Assays: Primary chromogenic substrate for quantifying enzyme activity in research and diagnostic kits.
• Antiviral Drug Screening: Used in high-throughput screening platforms to evaluate potential neuraminidase inhibitors for influenza and other viruses.
• Bacterial Pathogenesis Studies: Employed to study bacterial neuraminidases involved in host colonization and infection.
• Glycobiology Research: A key reagent for investigating sialic acid metabolism, cell surface glycan remodeling, and receptor function.
• Diagnostic Development: Integral component in the formulation of clinical and research-grade diagnostic tests for neuraminidase-producing organisms.
• Enzyme Kinetics & Characterization: Enables precise measurement of kinetic parameters (Km, Vmax) for neuraminidase enzymes.

Basic Information

Product Name	2-o-(p-Nitrophenyl)-α-D-n-Acetylneuraminic Acid, Sodium Salt, X Hydrate
CAS No.	123549-14-4
Molecular Formula	C17H21N2NaO11 • xH2O
Molecular Weight	452.35 g/mol (Anhydrous)
Synonyms	4-Nitrophenyl N-Acetyl-α-D-neuraminide, Sodium Salt; p-Nitrophenyl N-Acetyl-α-neuraminate Sodium Salt; Neu5Ac-α-(4-Nitrophenyl) Sodium Salt; NANA-α-pNP Na Salt; Neuraminidase Substrate (pNP); 2-O-(4-Nitrophenyl)-α-D-N-acetylneuraminic Acid Sodium Salt; 4-Nitrophenyl-α-N-acetylneuraminide Sodium Salt
EINECS	Contact for details

Quality Control

Our 2-o-(p-Nitrophenyl)-α-D-n-Acetylneuraminic Acid, Sodium Salt, X Hydrate is manufactured under strict quality systems to ensure batch-to-batch consistency and performance in sensitive enzymatic assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting key specifications such as assay, water content, and suitability for use as a neuraminidase substrate.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term storage, consider under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 95.0%
Assay (Enzymatic)	Suitable for neuraminidase activity detection
Water Content (KF)	≤ 10.0% (as X Hydrate)
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.