Description

Coelenterazine Fcp is a synthetic, cell-permeable analog of the marine luciferin coelenterazine, specifically engineered for enhanced stability and performance in bioluminescent applications. This high-purity compound is a critical reagent for researchers and developers requiring sensitive, quantitative detection of calcium ions and other cellular analytes. It is primarily utilized in the life sciences and pharmaceutical industries for advanced cellular imaging, high-throughput screening (HTS), and the development of bioluminescent reporter assays.

Application

• Bioluminescence Resonance Energy Transfer (BRET) Assays: Serves as a superior substrate for Renilla luciferase in BRET-based protein-protein interaction studies and intracellular signaling pathway analysis.
• Calcium Ion Sensing: Acts as the core luminophore in aequorin-based systems for real-time, non-invasive monitoring of intracellular calcium flux with high signal-to-noise ratios.
• High-Throughput Screening (HTS): Enables the development of robust, homogeneous assays for drug discovery targeting G-protein-coupled receptors (GPCRs) and other key therapeutic targets.
• In Vivo & In Vitro Imaging: Provides a stable light source for longitudinal bioluminescent imaging (BLI) in small animal models and live-cell imaging platforms.
• Reporter Gene Assays: Used as a standard substrate for quantifying gene expression driven by promoters linked to Renilla or Gaussian luciferase genes.
• ATP Detection & Metabolite Analysis: Functions in coupled enzyme assays for the sensitive detection of ATP, creatine kinase, and other metabolites.
• Environmental Monitoring Biosensors: Incorporated into engineered biological systems for the detection of specific pollutants or toxins.
• Apoptosis & Cytotoxicity Assays: Utilized in assays designed to measure caspase activity and other markers of cell health and death.

Basic Information

Product Name	Coelenterazine Fcp
CAS No.	123437-33-2
Molecular Formula	C26H21N3O2
Molecular Weight	407.47 g/mol
Synonyms	Coelenterazine f; DeepBlueC™; 2-Deoxycoelenterazine; 6-(4-Fluorophenyl)-2-[(4-hydroxyphenyl)methyl]-8-(phenylmethyl)-7H-imidazo[2,1-a]purin-7-one; 8-Benzyl-6-(4-fluorophenyl)-2-(4-hydroxybenzyl)imidazo[1,2-a]purin-7(1H)-one; Fcp Coelenterazine; Coelenterazine 400a; Coelenterazine Fluorophenyl
EINECS	Contact for details

Quality Control

Every batch of Coelenterazine Fcp is manufactured and tested under strict quality management systems to ensure the high purity and consistency required for sensitive research applications. Our products undergo rigorous analytical testing, including HPLC for purity and identity confirmation, to meet exacting industry standards. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below under an inert atmosphere. This product is strictly light-sensitive (protect from all light exposure), easily oxidized (store under inert atmosphere), and hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, ethanol; slightly soluble in aqueous buffers
Bioluminescent Activity	Tested in functional assay (Contact for details)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.