Description

Coelenterazine Cp is a synthetic, high-purity analog of the native coelenterazine chromophore, specifically engineered for enhanced stability and performance in bioluminescent applications. This compound is critical for researchers and developers requiring a reliable and sensitive substrate for Renilla luciferase (Rluc) and other marine luciferase-based assays. It is an essential reagent for the life sciences, pharmaceutical, and biotechnology industries, where it is used extensively in high-throughput screening (HTS), gene expression studies, in vivo imaging, and cellular reporter assays.

Application

• Bioluminescence Resonance Energy Transfer (BRET) Assays: A key substrate for quantifying protein-protein interactions and receptor activation in live cells.
• Reporter Gene Assays: Used with Renilla luciferase as an internal control or primary reporter for monitoring transcriptional activity and promoter studies.
• In Vivo Bioluminescent Imaging (BLI): Enables real-time, non-invasive tracking of tumor growth, gene expression, and cell migration in small animal models.
• High-Throughput Screening (HTS): Provides a sensitive and rapid readout for drug discovery campaigns targeting various biological pathways.
• ATP Detection & Cell Viability Assays: Utilized in kits for measuring cellular ATP levels as an indicator of metabolically active cells.
• Environmental Monitoring & Biosensors: Serves as a core component in engineered biosensors for detecting specific analytes or environmental toxins.

Basic Information

Product Name	Coelenterazine Cp
CAS No.	123437-25-2
Molecular Formula	C₂₆H₂₁N₃O₂
Molecular Weight	407.47 g/mol
Synonyms	Coelenterazine-cp; Cp-coelenterazine; 2-Deoxycoelenterazine; 6-(4-Methoxyphenyl)-2-[(4-methoxyphenyl)methyl]-8-phenylimidazo[1,2-a]pyrazin-3(7H)-one; Benzyl-coelenterazine; DeepBlueC™ substrate (trade name variant); Rluc substrate Cp
EINECS	Contact for details

Quality Control

Our Coelenterazine Cp is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot is characterized using advanced analytical techniques, including HPLC, LC-MS, and NMR, to confirm identity, purity, and the absence of critical impurities like native coelenterazine. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment. We support research and diagnostic applications requiring GMP-grade materials and can provide documentation to meet regulatory standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below under an inert atmosphere (argon or nitrogen). Under these conditions, the product is stable for at least 24 months. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize condensation and oxidative degradation. Aliquot into single-use vials after opening to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum conforms to structure
Purity (HPLC)	≥ 98.0% (Area %)
Water Content (KF)	≤ 1.0 %
Bioluminescent Activity	≥ 95% relative to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.