Description

Coelenterazine F CAS NO 123437-16-1 is a synthetic, cell-permeable luminogenic substrate derived from the natural coelenterazine molecule. This compound is essential for generating bioluminescent signals in various reporter gene assays and cellular imaging studies. It is primarily utilized by researchers and developers in the life sciences, pharmaceutical, and biotechnology industries for applications such as high-throughput screening, gene expression analysis, and in vivo imaging.

Application

• Bioluminescence Resonance Energy Transfer (BRET) Assays: A key substrate for studying protein-protein interactions and receptor-ligand binding in live cells.
• Reporter Gene Assays: Used with Renilla luciferase as a standard reporter system for monitoring gene expression, promoter activity, and signaling pathways.
• In Vivo Bioluminescent Imaging (BLI): Enables real-time, non-invasive tracking of tumor growth, metastasis, and therapeutic response in small animal models.
• High-Throughput Screening (HTS): Facilitates rapid and sensitive screening of drug candidates and bioactive compounds in automated systems.
• ATP Detection and Cell Viability Assays: Employed in sensitive assays for quantifying cellular ATP levels as an indicator of cell health and proliferation.
• Calcium Ion Sensing: Serves as a cofactor in engineered photoproteins like aequorin for measuring intracellular calcium flux.

Basic Information

Item	Detail
Product Name	Coelenterazine F
CAS No.	123437-16-1
Molecular Formula	C26H21N3O2
Molecular Weight	407.47 g/mol
Synonyms	Coelenterazine f; DeepBlueC™; 2-Deoxycoelenterazine; 8-Benzyl-2-(4-hydroxybenzyl)-6-(4-hydroxyphenyl)imidazo[1,2-a]pyrazin-3(7H)-one; 8-Benzyl-6-(4-hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl]imidazo[1,2-a]pyrazin-3(7H)-one; DBC; CLZN-f
EINECS	Contact for details

Quality Control

Our Coelenterazine F is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and MS to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay results, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below under an inert atmosphere. This product is strictly light-sensitive (protect from all light exposure) and easily oxidized (store under inert atmosphere). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Bioluminescence Activity	Conforms to performance standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.