Description

4-Nitrophenyl Phosphate Di(Cyclohexylamine) Salt Monohydrate is a high-purity chromogenic substrate widely used in enzymatic assays. This compound is critical for the detection and quantification of alkaline phosphatase activity, a key enzyme in diagnostic and research applications. It is an essential reagent for clinical diagnostics, life science research, and pharmaceutical quality control, where reliable and sensitive detection is paramount.

Application

• Clinical Diagnostics: Primary substrate for colorimetric detection of alkaline phosphatase (ALP) in ELISA kits and automated clinical chemistry analyzers.
• Life Science Research: Used in molecular biology and biochemistry for enzyme-linked immunosorbent assays (ELISAs), Western blotting, and other immunoassays.
• Pharmaceutical Testing: Employed in quality control and potency assays for biopharmaceuticals where ALP is used as a reporter enzyme.
• Food Safety & Environmental Monitoring: Applied in biosensor development and test kits for detecting bacterial contamination or specific analytes.
• Academic & Industrial R&D: Serves as a standard reagent in method development and high-throughput screening (HTS) platforms.

Basic Information

Product Name	4-Nitrophenyl Phosphate Di(Cyclohexylamine) Salt Monohydrate
CAS No.	123334-11-2
Molecular Formula	C24H46N3O7P
Molecular Weight	519.61 g/mol
Synonyms	p-Nitrophenyl Phosphate Di(Cyclohexylammonium) Salt Monohydrate; PNPP Di(Cyclohexylamine) Salt; 4-NPP Di(Cyclohexylamine) Salt; Phosphoric Acid 4-Nitrophenyl Ester Di(Cyclohexylamine) Salt Monohydrate; Alkaline Phosphatase Substrate; ALP Substrate; DiCyclohexylammonium p-Nitrophenyl Phosphate Monohydrate
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl Phosphate Di(Cyclohexylamine) Salt Monohydrate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance specifications. We ensure compliance with relevant standards for in-vitro diagnostic and research use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). Allow the sealed container to reach room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	3.0 - 5.0%
Solubility	Clear, colorless solution in water
Performance (Enzymatic)	Suitable for use as ALP substrate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.