Description

Phenolphthalein Diphosphate Tetrasodium Salt is a high-purity, water-soluble derivative of phenolphthalein, specifically designed as a chromogenic substrate for enzymatic assays. This compound is critical for the sensitive and quantitative detection of alkaline phosphatase (ALP) activity, a key biomarker in diagnostic and research applications. It is an essential reagent for clinical diagnostics, life science research, and quality control in the pharmaceutical and biotechnology industries, where precise enzymatic measurement is paramount.

Application

• Clinical Diagnostics: Primary substrate in colorimetric assays for measuring Alkaline Phosphatase (ALP) activity in serum, used for liver and bone disorder diagnosis.
• Immunoassays (ELISA): Chromogenic agent in enzyme-linked immunosorbent assays for detecting antibodies, antigens, or hormones.
• Molecular Biology Research: Used in blotting techniques (e.g., Western, Southern) for the detection of phosphatase-labeled probes.
• Pharmaceutical QC: Testing for phosphatase activity in drug manufacturing processes and final product sterility testing.
• Food Safety Testing: Detection of microbial contamination (e.g., *E. coli*) via phosphatase activity assays in dairy and other food products.
• Biochemical Research: Tool for studying enzyme kinetics, inhibition, and signal transduction pathways involving phosphatases.
• Histochemistry: Substrate for visualizing alkaline phosphatase activity in tissue sections for pathological studies.

Basic Information

Product Name	Phenolphthalein Diphosphate Tetrasodium Salt
CAS No.	123334-09-8
Molecular Formula	C20H12Na4O10P2
Molecular Weight	604.21 g/mol
Synonyms	Phenolphthalein Diphosphate Tetrasodium; Phenolphthalein Bisphosphate Tetrasodium Salt; PDP; Phenolphthalein Diphosphate (Tetrasodium Salt); 3,3-Bis(4-hydroxyphenyl)-1(3H)-isobenzofuranone Diphosphate Tetrasodium Salt; ALP Substrate; Alkaline Phosphatase Substrate; 4,4'-(3H-2,1-Benzoxathiol-3-ylidene)bis[2-(phosphonooxy)benzenesulfonic acid] S,S-Dioxide Tetrasodium Salt (obsolete nomenclature).
EINECS	Contact for details

Quality Control

Our Phenolphthalein Diphosphate Tetrasodium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectroscopy for identification, and stringent limits for moisture and heavy metals to ensure performance consistency and reliability. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory compliance (ISO, GMP) needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture, which may affect performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Water Content	≤ 2.0%
Solubility (1% in Water)	Clear, colorless to faint pink solution
Enzymatic Activity	Suitable for use as ALP substrate
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.