Description

H-D-Leu-Thr-Arg-Pna is a synthetic peptide substrate designed for high-precision biochemical research and diagnostic applications. This compound is critical for studying enzyme kinetics and protease activity, offering researchers a reliable tool for mechanistic investigations. It is primarily utilized by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic institutions focused on enzymology and proteomics.

Application

• Protease & Enzyme Assay Development: Used as a specific chromogenic substrate for measuring the activity of serine proteases and other hydrolytic enzymes.
• Pharmaceutical Research: Employed in high-throughput screening (HTS) campaigns to identify and characterize novel enzyme inhibitors for therapeutic development.
• Diagnostic Manufacturing: Serves as a key component in the formulation of clinical diagnostic kits for detecting specific enzymatic activity in biological samples.
• Biochemical Mechanism Studies: Facilitates detailed kinetic analysis and determination of catalytic efficiency (kcat/Km) for target enzymes.
• Quality Control in Biologics: Applied in purity and potency testing of enzyme-based biopharmaceutical products.

Basic Information

Product Name	H-D-Leu-Thr-Arg-Pna
CAS No.	122630-72-2
Molecular Formula	C24H39N9O7
Molecular Weight	565.63 g/mol
Synonyms	H-D-Leucyl-L-threonyl-L-arginine 4-nitroanilide; D-Leucyl-L-threonyl-L-arginine p-nitroanilide; Leu-Thr-Arg-pNA; L-Threonine, L-arginyl-D-leucyl-, 4-nitroanilide; Substrate for Thrombin and Trypsin; Chromogenic Substrate S-2366 (related analog); D-Leu-Thr-Arg-pNA; H-D-Leu-Thr-Arg-pNA
EINECS	Contact for details

Quality Control

Our H-D-Leu-Thr-Arg-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, identity confirmation by mass spectrometry (MS) and infrared spectroscopy (IR), and stringent controls for moisture and related substances. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to ensure full traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.