Description

Nhs-Peg-Nhs CAS NO 122375-06-8 is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring N-hydroxysuccinimide (NHS) esters at both termini. This compound is critical for creating stable amide bonds between amine-containing molecules, enabling precise and efficient bioconjugation. It is an essential reagent for researchers and manufacturers in biopharmaceutical development, diagnostic assay production, and advanced materials science, where controlled linker length and aqueous solubility are paramount.

Application

• Antibody-Drug Conjugate (ADC) Linker: Spacer for connecting cytotoxic payloads to monoclonal antibodies.
• Protein-Polymer Conjugation: PEGylation of therapeutic proteins to enhance stability and reduce immunogenicity.
• Biomaterial & Hydrogel Fabrication: Crosslinking agent for creating biocompatible scaffolds and networks.
• Diagnostic Probe Labeling: Conjugation of enzymes, dyes, or other reporters to antibodies and oligonucleotides for ELISA, flow cytometry, and imaging.
• Surface Functionalization: Immobilization of biomolecules onto chips, beads, or sensors via amine-reactive chemistry.
• Nanoparticle Modification: Surface coating and functionalization of liposomes and polymeric nanoparticles for drug delivery.

Basic Information

Product Name	Nhs-Peg-Nhs
CAS No.	122375-06-8
Molecular Formula	C2n+4H4n+6N2O2n+8 *
Molecular Weight	Variable (PEG chain length dependent)
Synonyms	Bis(N-succinimidyl) PEG ester; NHS-PEG-NHS; α,ω-Bis(N-succinimidyloxycarbonyl)polyethylene glycol; PEG-bis(NHS ester); Poly(ethylene glycol) bis(succinimidyl succinate); Succinimidyl PEG disuccinate; DSPE-PEG-NHS (for specific phospholipid conjugates); Bifunctional PEG NHS ester; PEG dithio-bis(succinimidyl propionate) analog (for cleavable variants).
EINECS	Contact for details

* Formula is representative. Exact formula depends on the number (n) of ethylene oxide repeating units in the PEG chain.

Quality Control

Our Nhs-Peg-Nhs is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and assay, NMR for structural confirmation, and functional group analysis to ensure consistent performance. Certificates of Analysis (COA) with detailed specifications are provided for every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (IR)	Conforms to structure
PEG Average MW (by NMR)	As ordered (e.g., 2kDa, 3.4kDa, 5kDa)
Assay (HPLC)	≥ 95%
Functional Substitution	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile; partially soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.