Description

5'-O-(4,4'-Dimethoxytrityl)-N2-dimethylaminomethylene-7-deaza-2'-deoxyguanosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound matters because it enables the precise and efficient incorporation of modified 7-deaza-2'-deoxyguanosine analogs into DNA sequences, which is critical for enhancing stability and altering hybridization properties. It is primarily needed by biotechnology and pharmaceutical companies engaged in advanced research, diagnostic assay development, and therapeutic oligonucleotide manufacturing.

Application

• Oligonucleotide Synthesis: As a key building block (phosphoramidite) in automated solid-phase DNA/RNA synthesizers for research-scale and GMP production.
• Antisense & siRNA Therapeutics: For the synthesis of modified oligonucleotides with improved nuclease resistance and binding affinity for therapeutic applications.
• PCR Primers & Probes: Manufacture of specialized primers and fluorescent probes containing 7-deazaguanosine for diagnostic assays and qPCR.
• Structural Biology Studies: Synthesis of oligonucleotides used in X-ray crystallography and NMR to study nucleic acid structure and protein interactions.
• Next-Generation Sequencing (NGS): Production of custom sequencing primers and adapters with modified bases to reduce sequencing artifacts.
• Aptamer Development: Creation of synthetic DNA libraries (SELEX) for discovering high-affinity aptamers against therapeutic targets.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-N2-dimethylaminomethylene-7-deaza-2'-deoxyguanosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite
CAS No.	121767-97-3
Molecular Formula	C46H55N8O7P
Molecular Weight	863.0 g/mol
Synonyms	7-Deaza-dG Phosphoramidite; DMT-d7G(ib) Phosphoramidite; 5'-O-DMT-N2-(dimethylaminomethylene)-7-deaza-2'-deoxyguanosine-3'-(2-cyanoethyl N,N-diisopropyl)phosphoramidite; N2-DMF-7-deaza-2'-deoxyguanosine phosphoramidite; c7dG Phosphoramidite; 7-Deaza-2'-deoxyguanosine, N2-protected phosphoramidite; Guanosine, 2'-deoxy-7-deaza-, 3'-[bis(1-methylethyl)amino](2-cyanoethoxy)phosphino]-5'-[bis(4-methoxyphenyl)phenylmethoxy]-N-[(dimethylamino)methylene]-
EINECS	Contact for details

Quality Control

Our 5'-O-DMT-7-deaza-dG phosphoramidite is manufactured under strict quality control protocols to ensure consistency and reliability for critical oligonucleotide synthesis. Each batch is characterized by HPLC, 1H/31P NMR, and mass spectrometry to confirm identity and high purity. We provide comprehensive Certificates of Analysis (COA) with detailed specifications, supporting use in research and cGMP environments. Our quality system is designed to meet the stringent demands of the pharmaceutical and diagnostic industries.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.