Description

Fluo 3-Am is a high-purity, cell-permeant fluorescent calcium indicator widely used in biological research. Its core value lies in its high affinity for calcium ions, enabling precise real-time measurement of intracellular calcium concentration ([Ca²⁺]i) dynamics. This compound is essential for researchers and professionals in neuroscience, pharmacology, and cell biology who require reliable tools for studying cellular signaling pathways, receptor function, and ion channel activity.

Application

• Intracellular Calcium Imaging: Primary tool for visualizing and quantifying real-time changes in cytosolic calcium levels in live cells.
• Neuroscience Research: Monitoring calcium transients in neurons and glial cells to study synaptic transmission, plasticity, and neurodegenerative processes.
• Drug Discovery & Pharmacology: High-throughput screening (HTS) assays to evaluate the effect of pharmaceutical compounds on calcium signaling pathways and GPCR activity.
• Cardiovascular Research: Investigating calcium handling in cardiomyocytes, which is critical for understanding heart muscle contraction and related diseases.
• Cell Physiology Studies: Probing calcium's role as a secondary messenger in processes like muscle contraction, secretion, gene expression, and apoptosis.
• Fluorescence-Activated Cell Sorting (FACS): Used as a fluorescent probe for sorting cell populations based on their calcium activity or loading efficiency.

Basic Information

Product Name	Fluo 3-Am
CAS No.	121714-22-5
Molecular Formula	C₅₁H₅₀F₂N₂O₂₃
Molecular Weight	1129.0 g/mol
Synonyms	Fluo-3 AM; Fluo-3 Acetoxymethyl Ester; Fluo 3 Acetoxymethyl ester; Fluo3 AM; Fluo3AM; 2-[6-[Bis(carboxymethyl)amino]-5-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid, acetoxymethyl ester; 1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]-2-(2-amino-5-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester
EINECS	Contact for details

Quality Control

Our Fluo 3-Am is manufactured under strict quality protocols to ensure batch-to-batch consistency and performance reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to meet exacting standards. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate and keep frozen. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Fluorescence Performance	Conforms to functional assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.