Description

Fluo 3 is a high-purity, cell-permeant fluorescent calcium indicator dye widely used in biological research. Its core value lies in its high selectivity and affinity for calcium ions (Ca²⁺), enabling precise real-time monitoring of intracellular calcium dynamics in live cells. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, particularly for applications in cell signaling studies, drug discovery, and neurobiological research.

Application

• Intracellular Calcium Imaging: Primary tool for visualizing and quantifying changes in cytosolic free Ca²⁺ concentration in live cells using fluorescence microscopy or flow cytometry.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) assays to evaluate the effect of pharmacological compounds on calcium signaling pathways.
• Neuroscience Research: Critical for studying calcium transients in neurons, synaptic activity, and mechanisms underlying neurological disorders.
• Cardiovascular Research: Employed to investigate calcium handling in cardiomyocytes and smooth muscle cells.
• Cell Physiology Studies: Facilitates research into calcium's role in processes like muscle contraction, secretion, gene expression, and apoptosis.
• Diagnostic Assay Development: Serves as a key component in the development of in vitro diagnostic (IVD) kits for cellular function analysis.

Basic Information

Product Name	Fluo 3
CAS No.	121714-13-4
Molecular Formula	C₅₁H₅₀Cl₂N₂O₂₃
Molecular Weight	1129.84 g/mol
Synonyms	Fluo-3; Fluo 3 pentapotassium salt; Fluo-3, pentapotassium salt; 2-[2-[2-[Bis(carboxymethyl)amino]-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy]ethoxy]-4-methylaniline-N,N,O-triacetic acid pentapotassium salt; 2-[2-[2-[Bis(2-carboxyatoethyl)amino]-5-(2,7-dichloro-6-oxido-3-oxo-3H-xanthen-9-yl)phenoxy]ethoxy]-4-methylaniline-N,N,O-triacetate pentapotassium; BAPTA-based Ca²⁺ indicator
EINECS	Contact for details

Quality Control

Our Fluo 3 is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. We provide a detailed Certificate of Analysis (COA) with every shipment, confirming compliance with our stringent in-house specifications suitable for sensitive life science applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorescence Properties	Ex/Em ~506/526 nm (bound to Ca²⁺)
Solubility	Soluble in aqueous buffer, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.