Description

n-(3-Iodo-4-Azidophenylpropionamido)Cysteinyl-5-(2'-Thiopyridyl)Cysteine-Coenzyme A is a sophisticated, bifunctional chemical probe designed for advanced biochemical research and labeling applications. This compound is critical for researchers requiring high-precision, site-specific modification of proteins and enzymes, particularly in proteomics and structural biology studies. Its unique structure, integrating a photoactivatable azide group and a cleavable disulfide linker, makes it an essential tool for scientists in pharmaceutical R&D, academic institutions, and biotechnology companies focused on target identification, mechanism-of-action studies, and activity-based protein profiling.

Application

• Activity-Based Protein Profiling (ABPP): Used as an active-site directed probe to label and identify enzymes, particularly those with reactive cysteine residues, within complex proteomes.
• Chemoproteomics and Target Engagement Studies: Enables the covalent capture and identification of drug targets and off-targets by mimicking natural coenzyme A substrates.
• Post-Translational Modification Analysis: Facilitates the study of protein acylation, such as palmitoylation, by serving as a chemical reporter or trapping agent.
• Enzyme Mechanism and Inhibition Studies: Acts as an affinity label to investigate enzyme active sites, kinetics, and the effects of potential inhibitors.
• Bioconjugation and Protein Labeling: Serves as a versatile intermediate for creating custom protein conjugates via click chemistry (via the azide) and disulfide exchange.
• Diagnostic Assay Development: Employed in the design of sensitive assays for detecting specific enzymatic activities relevant to disease biomarkers.

Basic Information

Product Name	n-(3-Iodo-4-Azidophenylpropionamido)Cysteinyl-5-(2'-Thiopyridyl)Cysteine-Coenzyme A
CAS No.	121368-75-0
Molecular Formula	C₃₈H₅₀IN₉O₁₇P₃S₂
Molecular Weight	1296.86 g/mol
Synonyms	3-Iodo-4-azidophenylpropionamido cysteinyl-5-(2'-thiopyridyl)cysteine-CoA; CoA-based ABPP probe; Iodo-azido phenylpropionamido-CoA probe; Activity-based probe for cysteine enzymes; Coenzyme A activity-based probe; 3-Iodo-4-azido-PP-CoA; Cysteine-targeted CoA probe; Azido-CoA affinity label
EINECS	Contact for details

Quality Control

Every batch of our n-(3-Iodo-4-Azidophenylpropionamido)Cysteinyl-5-(2'-Thiopyridyl)Cysteine-Coenzyme A is synthesized and purified under controlled conditions to ensure the highest standards of chemical purity and functional integrity. Quality is verified through a comprehensive suite of analytical techniques including HPLC, mass spectrometry, and NMR spectroscopy. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and suitability for research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. Due to its strictly light-sensitive and hygroscopic nature, this material must be handled under inert atmosphere or in a controlled, dry environment when aliquoting. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to theoretical mass
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Functional Activity	Tested in standard enzyme labeling assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.