Description

H-Ala-Phe-Lys-Amc Trifluoroacetate Salt is a high-purity, fluorophore-labeled peptide substrate designed for sensitive enzymatic assays. This compound matters for researchers and quality control labs requiring precise, reliable detection of specific protease activity. It is primarily needed by the pharmaceutical, biotechnology, and diagnostic industries for drug discovery, biochemical research, and assay development.

Application

• Protease Activity Assays: As a fluorogenic substrate for the detection and quantification of specific serine or cysteine protease activity.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize potential protease inhibitors.
• Enzyme Kinetics Studies: Enables detailed analysis of enzyme mechanisms, including determination of kinetic parameters (Km, Vmax).
• Biochemical Research: A critical tool for studying protease function in cellular processes, signaling pathways, and disease models.
• Diagnostic Kit Development: Serves as a key component in the development of clinical diagnostic tests for conditions involving dysregulated protease activity.
• Quality Control in Biologics: Used to monitor and ensure the consistency and activity of enzyme-based therapeutic products during manufacturing.

Basic Information

Product Name	H-Ala-Phe-Lys-Amc Trifluoroacetate Salt
CAS No.	120928-02-1
Molecular Formula	C27H34F3N5O6
Molecular Weight	605.59 g/mol
Synonyms	Ala-Phe-Lys-AMC TFA Salt; AFK-AMC TFA Salt; H-Ala-Phe-Lys-7-Amino-4-methylcoumarin Trifluoroacetate; Substrate for Plasmin; Fluorogenic Plasmin Substrate; L-Alanyl-L-phenylalanyl-L-lysine 7-amido-4-methylcoumarin trifluoroacetate salt; 7-Amino-4-methylcoumarin-labeled peptide substrate
EINECS	Contact for details

Quality Control

Our H-Ala-Phe-Lys-Amc Trifluoroacetate Salt is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.