Description

2-Chloro-4-Nitrophenyl-β-D-Lactoside is a specialized chromogenic substrate used in enzymatic assays and biochemical research. This compound is valued for its role in detecting and quantifying β-galactosidase activity, a critical enzyme in molecular biology and diagnostic applications. It is primarily utilized by research institutions, diagnostic kit manufacturers, and pharmaceutical companies involved in enzyme-linked assays and high-throughput screening.

Application

• Enzymatic Substrate for β-Galactosidase: Used as a chromogenic substrate in colorimetric assays to detect and measure β-galactosidase enzyme activity in research and diagnostics.
• Molecular Biology Research: Essential in reporter gene assays (e.g., lacZ gene expression) for studying gene regulation and promoter activity.
• Diagnostic Kit Manufacturing: Incorporated into commercial diagnostic kits for clinical microbiology and genetic testing to identify specific bacterial enzymes or genetic markers.
• High-Throughput Screening (HTS): Employed in pharmaceutical and biotech drug discovery platforms to screen for enzyme inhibitors or activators.
• Biochemical Reagent: Serves as a key reagent in biochemistry laboratories for enzyme kinetics studies and purity assessments.
• Cell Biology & Histochemistry: Used in staining procedures to visualize β-galactosidase expression in tissues and cell cultures.

Basic Information

Product Name	2-Chloro-4-Nitrophenyl-β-D-Lactoside
CAS No.	120583-41-7
Molecular Formula	C18H24ClNO13
Molecular Weight	497.84 g/mol
Synonyms	2-Chloro-4-nitrophenyl β-D-lactopyranoside; 2-Chloro-4-nitrophenyl β-D-galactopyranosyl-(1→4)-β-D-glucopyranoside; CNP-β-D-Lactoside; 4-Nitro-2-chlorophenyl β-lactoside; β-Galactosidase substrate CNP; Chromogenic β-galactosidase substrate; Lactose derivative chromogen; 2-Chloro-4-nitrophenyl lactoside
EINECS	Contact for details

Quality Control

Our 2-Chloro-4-Nitrophenyl-β-D-Lactoside is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, identity confirmation by IR and NMR, and validation of performance in enzymatic assays. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed immediately after use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Enzymatic Activity	Suitable for β-galactosidase assays
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.