Description

2',3'-O (2,4,6-Trinitrophenyl)-8-Azidoadenosine Diphosphate is a specialized, high-purity biochemical reagent designed for advanced research applications. This compound serves as a critical tool for studying nucleotide-dependent processes, offering unique photoreactive and affinity labeling capabilities. It is primarily utilized by researchers and scientists in pharmaceutical development, enzymology, and molecular biology for probing enzyme mechanisms and protein-nucleotide interactions.

Application

• Enzyme Mechanism Studies: As a substrate analog for investigating the active sites and catalytic mechanisms of kinases, polymerases, and other ATP/GTP-dependent enzymes.
• Affinity Labeling & Photoaffinity Labeling: The azido and trinitrophenyl groups enable covalent cross-linking to target proteins for identification and isolation.
• Biochemical Probe Development: Used in the synthesis of probes for detecting and quantifying specific nucleotide-binding proteins in complex mixtures.
• Structural Biology Research: Aids in X-ray crystallography and NMR studies by providing heavy-atom derivatives or specific binding ligands.
• Signal Transduction Research: Employed to study purinergic signaling pathways and the role of modified nucleotides in cellular regulation.
• Diagnostic Assay Development: Serves as a key component in developing high-sensitivity assays for clinical and research diagnostics.

Basic Information

Item	Detail
Product Name	2',3'-O (2,4,6-Trinitrophenyl)-8-Azidoadenosine Diphosphate
CAS No.	120218-68-0
Molecular Formula	C16H14N10O17P2
Molecular Weight	704.22 g/mol
Synonyms	8-Azido-2',3'-O-(2,4,6-trinitrophenyl)adenosine 5'-diphosphate; TNP-8-N3-ADP; 8-N3-TNP-ADP; 2',3'-O-(2,4,6-Trinitrophenyl)-8-azidoadenosine 5'-diphosphate; 8-Azidoadenosine-2',3'-O-(2,4,6-trinitrophenyl)diphosphate; Photoreactive TNP-ADP analog; 120218-68-0
EINECS	Contact for details

Quality Control

Every batch of 2',3'-O (2,4,6-Trinitrophenyl)-8-Azidoadenosine Diphosphate is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and consistency. Our quality assurance protocol includes advanced chromatographic and spectroscopic methods. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with each shipment to support your research integrity and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) due to its sensitivity to moisture and oxidation. Allow the container to reach room temperature before opening to minimize condensation and exposure to ambient humidity. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay	≥ 90% (by UV-Vis)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.